Production purification and fecal fermentation of fructooligosaccharide by FTase from Jerusalem artichoke

P. Prakobpran, . and S. Wichienchot, . and B. Ngampanya, . and P. Jaturapiree, . Production purification and fecal fermentation of fructooligosaccharide by FTase from Jerusalem artichoke. pp. 134-141. ISSN ISSN(Online):22317546

Fructooligosaccharides (FOS) has been used as prebiotic that serves as a substrate for microflora in the large intestine. FOS are produced by fructosyltransferase (FTase) derived from some plants such as Jerusalem artichoke chicory asparagus banana dragon fruit and onion. It was found that Jerusalem artichoke cultured in tropical region for 3-5 months showed good source of FTase. It had the highest crude enzyme activity of 0.2530.003 U/ml. Optimal conditions for purification of FTase by chromatography techniques with anion exchangers showed the highest specific activity which increased from 1.411 to 2.240 U/ml. Optimum conditions for production of FOS were 20 sucrose reaction time of 96 h and 1 U/ml FTase. It was found that highest FOS (35) consisted of 27.5 1-kestose (DP 2) and 7.5 nystose (DP 3). Fructooligosaccharide was further purified by yeast fermentation using 2.5 Saccharomyces cerevisiae TISTR5019 for 36 h. It could decrease sucrose from 46.1 to 28.7. The chemical composition of partially purified freeze-dried FOS was analyzed by HPLC. It consisted of 35 FOS 17.4 sucrose 7.6 glucose and 40 fructose. The prebiotic property of the partially purified FOS was evaluated by cultivation of human fecal microflora in batch culture. It was found that the FOS had prebiotic property with PI value of 2.33. There was also stimulated growth of Lactobacillus and Bifidobacterium. It also produced lactic acid acetic acid propionic acid and butyric acid at concentration of 1287.92 20.97 10.34 and 18.67 g/ml respectively and vitamins B1 and folic acid were produced at concentration of 19.19 and 20.00 g/ml respectively.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(01)%202017/(16).p...

Abstract

Fructooligosaccharides (FOS) has been used as prebiotic that serves as a substrate for microflora in the large intestine. FOS are produced by fructosyltransferase (FTase) derived from some plants such as Jerusalem artichoke chicory asparagus banana dragon fruit and onion. It was found that Jerusalem artichoke cultured in tropical region for 3-5 months showed good source of FTase. It had the highest crude enzyme activity of 0.2530.003 U/ml. Optimal conditions for purification of FTase by chromatography techniques with anion exchangers showed the highest specific activity which increased from 1.411 to 2.240 U/ml. Optimum conditions for production of FOS were 20 sucrose reaction time of 96 h and 1 U/ml FTase. It was found that highest FOS (35) consisted of 27.5 1-kestose (DP 2) and 7.5 nystose (DP 3). Fructooligosaccharide was further purified by yeast fermentation using 2.5 Saccharomyces cerevisiae TISTR5019 for 36 h. It could decrease sucrose from 46.1 to 28.7. The chemical composition of partially purified freeze-dried FOS was analyzed by HPLC. It consisted of 35 FOS 17.4 sucrose 7.6 glucose and 40 fructose. The prebiotic property of the partially purified FOS was evaluated by cultivation of human fecal microflora in batch culture. It was found that the FOS had prebiotic property with PI value of 2.33. There was also stimulated growth of Lactobacillus and Bifidobacterium. It also produced lactic acid acetic acid propionic acid and butyric acid at concentration of 1287.92 20.97 10.34 and 18.67 g/ml respectively and vitamins B1 and folic acid were produced at concentration of 19.19 and 20.00 g/ml respectively.

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Item Type:	Article
AGROVOC Term:	Fructose
AGROVOC Term:	Plants
AGROVOC Term:	Jerusalem artichokes
AGROVOC Term:	Fermentation
AGROVOC Term:	Enzymes
AGROVOC Term:	Yeasts
AGROVOC Term:	Lactic acid
AGROVOC Term:	Acetic acid
AGROVOC Term:	Propionic acid
AGROVOC Term:	Vitamin B1
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23256

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