Non-haemolytic enterotoxigenic Bacillus cereus strains from raw and pasteurized milk and milking utensils in Kelantan Malaysia

Aklilu E., . and Nur Atiqah R., . Non-haemolytic enterotoxigenic Bacillus cereus strains from raw and pasteurized milk and milking utensils in Kelantan Malaysia. pp. 401-405. ISSN ISSN(Online):22317546

The toxigenic strains of Bacillus cereus (B. cereus) produce toxins such as haemolytic and nonhaemolytic toxins. Fresh milk pasteurized milk and swab samples were collected from milking utensils in a diary farm. Routine microbiological examination for B. cereus antimicrobial sensitivity tests and PCR detection of the Bacillus group specific genes and genes encoding for haemolytic enterotoxin and non-hemolytic eneterotoxin genes were conducted. Bacillus cereus was isolated from raw bulk mlik pasteurized milk and milking utensils swabs collected. Detection of B. cereus was higher in milking utensils compared to raw and pasteurized milk. Bacillus cereus was also detected in corn-flavoured pasteurized milk and milking utensils of dairy colonies. There were non-hemolytic enterotoxin gene positive isolates and most of them were susceptible to Gentamicin Chloramphenicol and Ciprofloxacin compare to other beta lactam antibiotics. As control and prevention stratgies increase in public awareness through public education proper hygiene practices in farms and dairy processing plants regular surveillance and quality control including intensive screening for milk and milk products need to be in place. However further in-depth study based on larger and diversified sample and detection of other toxigenic genes are recommended.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(01)%202017/(53).p...

Abstract

The toxigenic strains of Bacillus cereus (B. cereus) produce toxins such as haemolytic and nonhaemolytic toxins. Fresh milk pasteurized milk and swab samples were collected from milking utensils in a diary farm. Routine microbiological examination for B. cereus antimicrobial sensitivity tests and PCR detection of the Bacillus group specific genes and genes encoding for haemolytic enterotoxin and non-hemolytic eneterotoxin genes were conducted. Bacillus cereus was isolated from raw bulk mlik pasteurized milk and milking utensils swabs collected. Detection of B. cereus was higher in milking utensils compared to raw and pasteurized milk. Bacillus cereus was also detected in corn-flavoured pasteurized milk and milking utensils of dairy colonies. There were non-hemolytic enterotoxin gene positive isolates and most of them were susceptible to Gentamicin Chloramphenicol and Ciprofloxacin compare to other beta lactam antibiotics. As control and prevention stratgies increase in public awareness through public education proper hygiene practices in farms and dairy processing plants regular surveillance and quality control including intensive screening for milk and milk products need to be in place. However further in-depth study based on larger and diversified sample and detection of other toxigenic genes are recommended.

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Item Type:	Article
AGROVOC Term:	Bacillus cereus
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	Antimicrobials
AGROVOC Term:	Isolation
AGROVOC Term:	Identification
AGROVOC Term:	Raw milk
AGROVOC Term:	Pasteurized milk
AGROVOC Term:	Milking equipment
AGROVOC Term:	Antibiotics
AGROVOC Term:	Contamination
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 03:30
URI:	http://webagris.upm.edu.my/id/eprint/23285

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