Citation
Soedjanaatmadja Ukun M. S., . and Subroto Toto, . and Beintema Jaap J., . and Soedigdo Soekeni, . Does hevein stabilise or destabilise rubber latex. pp. 69-77. ISSN 1511-1768
Abstract
The acidic protein hevein is the major protein in the lutoid-body fraction of rubber latex. The purified protein has a stabilising effect on suspensions of rubber particles in the presence of unfractionated lutoid-body preparations which contain destabilising basic proteins. The hevein contents in lutoid-body fractions from rubber clones GT1 and PR 261 are about two times higher than in that of clone LCB 1320 which may explain the faster destabilisation of latex from the latter clone after tapping. Hevein and pseudo-hevein bind chitin and can be isolated by affinity chromatography on a chitin column. Pseudo-hevein binds less strongly to this material than hevein which can be explained by a replacement of a tryptophan residue by tyrosine in the carbohydrate-binding site of the protein.
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Abstract
The acidic protein hevein is the major protein in the lutoid-body fraction of rubber latex. The purified protein has a stabilising effect on suspensions of rubber particles in the presence of unfractionated lutoid-body preparations which contain destabilising basic proteins. The hevein contents in lutoid-body fractions from rubber clones GT1 and PR 261 are about two times higher than in that of clone LCB 1320 which may explain the faster destabilisation of latex from the latter clone after tapping. Hevein and pseudo-hevein bind chitin and can be isolated by affinity chromatography on a chitin column. Pseudo-hevein binds less strongly to this material than hevein which can be explained by a replacement of a tryptophan residue by tyrosine in the carbohydrate-binding site of the protein.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Latex |
| AGROVOC Term: | Protein content |
| AGROVOC Term: | Freezing |
| AGROVOC Term: | Thawing |
| AGROVOC Term: | Lyophilization |
| AGROVOC Term: | Centrifugation |
| AGROVOC Term: | Ammonium sulphate |
| AGROVOC Term: | Stability |
| AGROVOC Term: | Rubber |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:28 |
| URI: | http://webagris.upm.edu.my/id/eprint/23292 |
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