Citation
Kaplan Deborah A., . and Lytle C. David, . and Routson Licia B., . and Myers Matthew R., . Issues associated with using fluorescent microspheres to evaluate the barrier integrity of natural rubber latex films. pp. 131-139. ISSN 1511-1768
Abstract
It has been reported that 100 nm - 110 nm fluorescent microspheres can pass through more natural rubber (NR) latex condoms than can the 27 nm virus fX174 as found by other investigators. Are there properties of fluorescent polystyrene microspheres that could be responsible for these disparate results This study demonstrated that the microspheres __ adsorbed to a brand of condom made of NR latex as well as a highly-adsorptive virus PRD1 (fX174 did not adsorb). The study also found that free fluorescent dye was not released when the microspheres were in close contact with the NR latex. These findings argue against microsphere properties being responsible for the disparate results. In addition an increase in the fluorescence of buffer after contact with NR latex condom was found in the absence of microspheres. This increase may require extended contact. Thus this study found an artifact that could be misconstrued as evidence of fluorescent microspheres passing through NR latex barriers.
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Abstract
It has been reported that 100 nm - 110 nm fluorescent microspheres can pass through more natural rubber (NR) latex condoms than can the 27 nm virus fX174 as found by other investigators. Are there properties of fluorescent polystyrene microspheres that could be responsible for these disparate results This study demonstrated that the microspheres __ adsorbed to a brand of condom made of NR latex as well as a highly-adsorptive virus PRD1 (fX174 did not adsorb). The study also found that free fluorescent dye was not released when the microspheres were in close contact with the NR latex. These findings argue against microsphere properties being responsible for the disparate results. In addition an increase in the fluorescence of buffer after contact with NR latex condom was found in the absence of microspheres. This increase may require extended contact. Thus this study found an artifact that could be misconstrued as evidence of fluorescent microspheres passing through NR latex barriers.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Natural rubber |
| AGROVOC Term: | Latex |
| AGROVOC Term: | Adsorption |
| AGROVOC Term: | Fluorescence |
| AGROVOC Term: | Measurement |
| AGROVOC Term: | Phosphates |
| AGROVOC Term: | Viruses |
| AGROVOC Term: | Buffers |
| AGROVOC Term: | Bacteriophages |
| AGROVOC Term: | Ultrafiltration |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:28 |
| URI: | http://webagris.upm.edu.my/id/eprint/23297 |
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