Genetic relatedness and identities of cultivated Hevea clones determined by isozymes

Napi D., . and Sunderasan E., . and Zamri A. S. M., . and Yeang H. Y., . and Wickneswari R., . Genetic relatedness and identities of cultivated Hevea clones determined by isozymes. pp. 35-47. ISSN 1511-1768

Isozyme markers were used to determine genetic relatedness between sixty cultivated Hevea clones originating from the Wickham collection. The mean observed heterozygosity for the clones was 0.567. A dendrogram representing phenetic relationships among the clones in the study was constructed using the unweighted pair-group method with arithmetic averaging. Considerable genetic diversity was observed (mean genetic similarity 0.754) despite the fact that the gene pool was derived from a small number of plants originally sourced from a single collection area in Brazil. Principal component analysis of the genetic diversity among the sixty clones showed that PR clones and PB clones formed distinctly different clusters. On the other hand the RRIM clones were evenly distributed reflecting the selection of RRIM clones from a generally wider genetic base. Isozyme polymorphism was evaluated as a tool for clonal identification and verification among source bushes (from which buds for vegetative propagation are generally obtained). A panel of seven isozymes identified fifty-two out of sixty commercially planted clones outright while four pairs of clones shared common zymograms. A 0.2 probability of identification error was estimated for the chance occurrence that a rogue plant would have an isozyme profile identical to that of the purported clone. Isozymes were also used to validate 24 clonal progenies derived from hand-pollination. Genotypes of 23 clones were found to be consistent with those of their assigned pedigrees. The exception was RRIM 936 which had some alleles that were absent in either of the purported parents.

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Abstract

Isozyme markers were used to determine genetic relatedness between sixty cultivated Hevea clones originating from the Wickham collection. The mean observed heterozygosity for the clones was 0.567. A dendrogram representing phenetic relationships among the clones in the study was constructed using the unweighted pair-group method with arithmetic averaging. Considerable genetic diversity was observed (mean genetic similarity 0.754) despite the fact that the gene pool was derived from a small number of plants originally sourced from a single collection area in Brazil. Principal component analysis of the genetic diversity among the sixty clones showed that PR clones and PB clones formed distinctly different clusters. On the other hand the RRIM clones were evenly distributed reflecting the selection of RRIM clones from a generally wider genetic base. Isozyme polymorphism was evaluated as a tool for clonal identification and verification among source bushes (from which buds for vegetative propagation are generally obtained). A panel of seven isozymes identified fifty-two out of sixty commercially planted clones outright while four pairs of clones shared common zymograms. A 0.2 probability of identification error was estimated for the chance occurrence that a rogue plant would have an isozyme profile identical to that of the purported clone. Isozymes were also used to validate 24 clonal progenies derived from hand-pollination. Genotypes of 23 clones were found to be consistent with those of their assigned pedigrees. The exception was RRIM 936 which had some alleles that were absent in either of the purported parents.

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Item Type:	Article
AGROVOC Term:	Hevea brasiliensis
AGROVOC Term:	Clonal variation
AGROVOC Term:	Genetic diversity within species
AGROVOC Term:	Genetic variation
AGROVOC Term:	Genetic markers
AGROVOC Term:	Identification
AGROVOC Term:	Cultivars
AGROVOC Term:	Isozymes
AGROVOC Term:	Buffers
AGROVOC Term:	Data analysis
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 03:30
URI:	http://webagris.upm.edu.my/id/eprint/23311

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