Citation
Azizah M., . and Shahnaz M., . and Mok K. L., . and Hasma H., . and Esah Yip, . and Nasuruddin B. A., . Binding patterns of IgE antibodies in sera of rubber tappers to fresh Hevea latex serum proteins. pp. 146-153. ISSN 1511-1768
Abstract
The binding patterns of lgE antibodies to fresh natural rubber latex B- and C-serum proteins were determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis immunoblotting technique. All the IgE samples were from Malaysian rubber tappers who had been skin-prick tested with extracts of gloves and food: avocado potato tomato and watermelon. Two of the five IgE samples from tappers reacting to gloves bound to latex proteins specifically to 35 38 and 40 kD B-serum proteins and to 30 kD and 75 kD C-serum proteins. The remaining three either did not bind or bound faintly to the latex proteins. Similar binding pattern to only a few latex proteins was shown in one of the three sera of tappers reacting to both gloves and food. The other two however exhibited multiple bindings to a wide variety of B-serum proteins of molecular weights less-than 20 kD to greater than 202 kD and to a number of C-serum proteins between 30 kD to 75 kD. The heterogeneous binding pattern was also demonstrated by eleven of the twenty IgE serum samples of tappers reacting to food and by twenty-five of the hundred and thirty-six serum samples of tappers reacting negatively to both gloves and food. The fact that only two of the thirty-eight serum samples that showed strong multiple binding pattern corresponded to a positive skin-prick test to gloves indicated that in vitro immunoblotting using IgE antibodies in human blood is an unreliable indicator of latex allergy.
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Abstract
The binding patterns of lgE antibodies to fresh natural rubber latex B- and C-serum proteins were determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis immunoblotting technique. All the IgE samples were from Malaysian rubber tappers who had been skin-prick tested with extracts of gloves and food: avocado potato tomato and watermelon. Two of the five IgE samples from tappers reacting to gloves bound to latex proteins specifically to 35 38 and 40 kD B-serum proteins and to 30 kD and 75 kD C-serum proteins. The remaining three either did not bind or bound faintly to the latex proteins. Similar binding pattern to only a few latex proteins was shown in one of the three sera of tappers reacting to both gloves and food. The other two however exhibited multiple bindings to a wide variety of B-serum proteins of molecular weights less-than 20 kD to greater than 202 kD and to a number of C-serum proteins between 30 kD to 75 kD. The heterogeneous binding pattern was also demonstrated by eleven of the twenty IgE serum samples of tappers reacting to food and by twenty-five of the hundred and thirty-six serum samples of tappers reacting negatively to both gloves and food. The fact that only two of the thirty-eight serum samples that showed strong multiple binding pattern corresponded to a positive skin-prick test to gloves indicated that in vitro immunoblotting using IgE antibodies in human blood is an unreliable indicator of latex allergy.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Rubber |
| AGROVOC Term: | Latex |
| AGROVOC Term: | Hevea |
| AGROVOC Term: | Proteins |
| AGROVOC Term: | Binding proteins |
| AGROVOC Term: | Allergens |
| AGROVOC Term: | Allergic responses |
| AGROVOC Term: | Antibodies |
| AGROVOC Term: | Centrifugation |
| AGROVOC Term: | Polyacrylamide gel electrophoresis |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 03:30 |
| URI: | http://webagris.upm.edu.my/id/eprint/23320 |
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