Citation
Esah Yip, . Measurements of total extractable proteins in latex gloves: a comparative study of the RRIM and ASTM tests. pp. 166-175. ISSN 0127-7065
Abstract
Extractable protein contents of latex gloves generated by two commonly used methods the RRIM (MS 1392:96P) and the ASTM (D 5712-95) modified Lowry tests were examined and their relationship studied. Total extractable proteins EPRRJM determined by the RRIM test ranged from 1326 m/g to 20 mg/g for 90 gloves. Their corresponding EPASTM values obtained by the ASTM test varied from 1377 mg/g to 50 mg/g. Statistical analysis showed a very significant correlation between them with a coefficient of correlation r 0.93 P 0.001. Generally EPRRJM values read higher than those of EPASTM. EPASTM of 50 mg/g and lower were found to be associated with EPRRJM values ranging from 267 mg/g to 20 mg/g suggesting higher sensitivity of the latter measurements. Relevance of the two sets of EP in relation to the allergenicity/allergic potential of latex gloves was discussed. Accelerated ageing at 70Cfor 7 days of latex gloves resulted in the lowering of protein contents. The effect appeared to be more pronounced for EPRRJM than for EPASTM.
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Abstract
Extractable protein contents of latex gloves generated by two commonly used methods the RRIM (MS 1392:96P) and the ASTM (D 5712-95) modified Lowry tests were examined and their relationship studied. Total extractable proteins EPRRJM determined by the RRIM test ranged from 1326 m/g to 20 mg/g for 90 gloves. Their corresponding EPASTM values obtained by the ASTM test varied from 1377 mg/g to 50 mg/g. Statistical analysis showed a very significant correlation between them with a coefficient of correlation r 0.93 P 0.001. Generally EPRRJM values read higher than those of EPASTM. EPASTM of 50 mg/g and lower were found to be associated with EPRRJM values ranging from 267 mg/g to 20 mg/g suggesting higher sensitivity of the latter measurements. Relevance of the two sets of EP in relation to the allergenicity/allergic potential of latex gloves was discussed. Accelerated ageing at 70Cfor 7 days of latex gloves resulted in the lowering of protein contents. The effect appeared to be more pronounced for EPRRJM than for EPASTM.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Latex |
| AGROVOC Term: | Natural rubber |
| AGROVOC Term: | Extraction |
| AGROVOC Term: | Centrifugation |
| AGROVOC Term: | Precipitation |
| AGROVOC Term: | Acid precipitation |
| AGROVOC Term: | Standards |
| AGROVOC Term: | Measurement |
| AGROVOC Term: | Ageing |
| AGROVOC Term: | Protein content |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 03:56 |
| URI: | http://webagris.upm.edu.my/id/eprint/23346 |
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