Recent advances in the development of molecular markers for Hevea studies

Low F. C., . and Safiah Atan, . and Hafsah Jaafar, . and Tan H., . Recent advances in the development of molecular markers for Hevea studies. pp. 32-44. ISSN 0127-7065

Four DNA molecular marker techniques were developed and applied to Hevea studies. These included the conventional Southern-based marker technique called restriction fragment length polymorphisms (RFLPs) and three polymerase chain reaction amplification-based techniques viz. random amplified polymorphic DNA microsatellites and DNA amplification fingerprinting (DAF). These molecular markers were able to elicit interspecific as well as intraspecific polymorphisms. They were also able to assist in several identification studies. Cultivars which share two common parents (siblings) such as PR 255 and PR 261; RRIM 901 and RRIM 905; and RRIM 93 7 and RRIM 938 were distinguished by their DNA polymorphisms. Similarly molecular markers had assisted the identification of rogues in a sample of monoclonal PB 5/51 seedlings. The genetic diversity of genotypes from the 1981 IRRDB Hevea germplasm collection from Brazil was displayed and confirmed by their RFLPs. For the first time microsatellites had been detected in the Hevea genome. Three microsatellite sequences were detected within one gene (hydroxylmethyl-glutaryl coenzyme A reductase-1). Preliminary evidence suggested that one of these microsatellite sequences (GA)9 was polymorphic. A reduction in the number of ribosomal DNA (rDNA) genes during cell culture was suggested when pTa71 a wheat rDNA probe was hybridised with digested gDNA obtained from in vitro culture plants. Somaclonal variations were detected among a population of GL 1 plants derived from anther and ovule culture by a recently developed DNA fingerprinting technique called DAF. A genetic linkage map comprising of RFLPs and other markers is being constructed. The advantages and disadvantages of these marker techniques were compared.

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Abstract

Four DNA molecular marker techniques were developed and applied to Hevea studies. These included the conventional Southern-based marker technique called restriction fragment length polymorphisms (RFLPs) and three polymerase chain reaction amplification-based techniques viz. random amplified polymorphic DNA microsatellites and DNA amplification fingerprinting (DAF). These molecular markers were able to elicit interspecific as well as intraspecific polymorphisms. They were also able to assist in several identification studies. Cultivars which share two common parents (siblings) such as PR 255 and PR 261; RRIM 901 and RRIM 905; and RRIM 93 7 and RRIM 938 were distinguished by their DNA polymorphisms. Similarly molecular markers had assisted the identification of rogues in a sample of monoclonal PB 5/51 seedlings. The genetic diversity of genotypes from the 1981 IRRDB Hevea germplasm collection from Brazil was displayed and confirmed by their RFLPs. For the first time microsatellites had been detected in the Hevea genome. Three microsatellite sequences were detected within one gene (hydroxylmethyl-glutaryl coenzyme A reductase-1). Preliminary evidence suggested that one of these microsatellite sequences (GA)9 was polymorphic. A reduction in the number of ribosomal DNA (rDNA) genes during cell culture was suggested when pTa71 a wheat rDNA probe was hybridised with digested gDNA obtained from in vitro culture plants. Somaclonal variations were detected among a population of GL 1 plants derived from anther and ovule culture by a recently developed DNA fingerprinting technique called DAF. A genetic linkage map comprising of RFLPs and other markers is being constructed. The advantages and disadvantages of these marker techniques were compared.

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Item Type:	Article
AGROVOC Term:	Hevea
AGROVOC Term:	Plant breeding
AGROVOC Term:	Molecular markers
AGROVOC Term:	Genetic markers
AGROVOC Term:	Genetic variation
AGROVOC Term:	Biotechnology
AGROVOC Term:	Identification
AGROVOC Term:	Cultivars
AGROVOC Term:	Hevea brasiliensis
AGROVOC Term:	Hybridization
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23357

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