Citation
Shamsul Bahri A. R., . and Samsidar Hamzah, . Immunocytochemical localisation of rubber membrane protein in Hevea latex. pp. 88-95. ISSN 0127-7065
Abstract
Immunogold double labelling technique by electron microscopy was employed to localise the 14 kDa and 24 kDa protein obtained from rubber particle membrane in Hevea latex. These proteins were used to elicit antibodies in rabbits. The two antibodies showed selective labelling on the rubber particles: 14 kDa antibody bound more to the larger rubber particles (0.3 mm) whereas the 24 kDa antibody more to the smaller rubber particles (0.2mm). The use of osmium tetroxide to fix rubber particles did not destroy the antigenic sites.
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Abstract
Immunogold double labelling technique by electron microscopy was employed to localise the 14 kDa and 24 kDa protein obtained from rubber particle membrane in Hevea latex. These proteins were used to elicit antibodies in rabbits. The two antibodies showed selective labelling on the rubber particles: 14 kDa antibody bound more to the larger rubber particles (0.3 mm) whereas the 24 kDa antibody more to the smaller rubber particles (0.2mm). The use of osmium tetroxide to fix rubber particles did not destroy the antigenic sites.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Rubber |
| AGROVOC Term: | Hevea brasiliensis |
| AGROVOC Term: | Membranes |
| AGROVOC Term: | Immunocytochemistry |
| AGROVOC Term: | Immunoblot analysis |
| AGROVOC Term: | Centrifugation |
| AGROVOC Term: | Fractionation |
| AGROVOC Term: | Immunoblotting |
| AGROVOC Term: | Particle size |
| AGROVOC Term: | Proteins |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:28 |
| URI: | http://webagris.upm.edu.my/id/eprint/23362 |
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