Citation
Safiah A., . and Low F. C., . and Saleh N. M., . Construction of a microsatellite-enriched library from Hevea brasiliensis. pp. 247-255. ISSN 0127-7065
Abstract
A microsatellite library was constructed from Hevea brasiliensis by cloning DNA fragments of between 200 bp - 800 bp in length. These were ligated to pBluescript KS phagemid as the vector and transformed into Escherichia coli DH5a. The library appeared to be highly enriched with simple sequence repeats; (GACA)n - 10 (GATA) n - 9 (GA) n - 34 and (GC)n -9.
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Abstract
A microsatellite library was constructed from Hevea brasiliensis by cloning DNA fragments of between 200 bp - 800 bp in length. These were ligated to pBluescript KS phagemid as the vector and transformed into Escherichia coli DH5a. The library appeared to be highly enriched with simple sequence repeats; (GACA)n - 10 (GATA) n - 9 (GA) n - 34 and (GC)n -9.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Hevea brasiliensis |
| AGROVOC Term: | Microsatellites |
| AGROVOC Term: | DNA cloning |
| AGROVOC Term: | Escherichia coli |
| AGROVOC Term: | Polymorphism |
| AGROVOC Term: | Hybridization |
| AGROVOC Term: | Genetic maps |
| AGROVOC Term: | Breeding |
| AGROVOC Term: | Molecular markers |
| AGROVOC Term: | Genomes |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 03:57 |
| URI: | http://webagris.upm.edu.my/id/eprint/23374 |
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