Citation
Ward M. A., . and Samsidar Hamzah, . and Yeang H. Y., . and Sunderasan E., . and Sharifah Hamid, . and Cardosa M. J., . Latex B-serum b-1 3-glucanase (Hev b II) and a component of the microhelix (Hev b IV) are major latex allergens. pp. 82-99. ISSN 0127-7065
Abstract
Two allergenic (IgE-binding) proteins have been isolated from the B-serum of natural rubber latex by dialysis-induced protein precipitation followed by gel filtration. One of the allergens Hev b II that is specific to the monoclonal antibody USM/RB4 commonly appears as a doublet of molecular weights 34 and 36 kDa under reducing conditions of SDS-polyacrylamide gel electrophoresis. In the absence of 2-mercaptoethanol a 70 kDa band can also be seen in addition to the doublet. The allergen has a pi of circa 9.5 and is identified as b-l 3-glucanase. The second allergen Hev b IV has a pI at circa 4.5 and appears under reducing conditions as a band of 50-57 kDa. In its unreduced form a principal band of approximately 100 kDa is discernible. Immuno-gold labelling with the monoclonal antibody USM/RB3 shows that Hev b IV is a component of the microhelix protein complex of B-serum.
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Abstract
Two allergenic (IgE-binding) proteins have been isolated from the B-serum of natural rubber latex by dialysis-induced protein precipitation followed by gel filtration. One of the allergens Hev b II that is specific to the monoclonal antibody USM/RB4 commonly appears as a doublet of molecular weights 34 and 36 kDa under reducing conditions of SDS-polyacrylamide gel electrophoresis. In the absence of 2-mercaptoethanol a 70 kDa band can also be seen in addition to the doublet. The allergen has a pi of circa 9.5 and is identified as b-l 3-glucanase. The second allergen Hev b IV has a pI at circa 4.5 and appears under reducing conditions as a band of 50-57 kDa. In its unreduced form a principal band of approximately 100 kDa is discernible. Immuno-gold labelling with the monoclonal antibody USM/RB3 shows that Hev b IV is a component of the microhelix protein complex of B-serum.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Hevea brasiliensis |
| AGROVOC Term: | Rubber crops |
| AGROVOC Term: | Latex |
| AGROVOC Term: | Allergens |
| AGROVOC Term: | Dialysis |
| AGROVOC Term: | Precipitation |
| AGROVOC Term: | Cytoplasm |
| AGROVOC Term: | Centrifugation |
| AGROVOC Term: | Organelles |
| AGROVOC Term: | Column chromatography |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:28 |
| URI: | http://webagris.upm.edu.my/id/eprint/23384 |
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