Citation
Yeang H. Y., . and Sunderasan E., . and Shamsul Bahri A. R., . New approaches in the quantitation of total proteins from latex gloves. pp. 70-78. ISSN 0127-7065
Abstract
Aspects of protein purification/concentration and detection were modified to enhance sensitivity rapidity and practicality in assaying total proteins from latex gloves. In purifying the eluted proteins by acid precipitation a combination of 5 trichloroacetic acid and 0.2 phosphotungstic acid was found to precipitate proteins most effectively. Maximal colour development in the Lowry micro-assay for proteins was attained when the protein precipitate was re-dissolved in 0.25M sodium hydroxide and when 6 Folin reagent was utilised in the reaction. As a simpler alternative to the colorimetric assay precipitated proteins were quantitated by UV absorption at 280 nm. The UV readings correlated well with results obtained by the Lowry assay. In another approach towards rapid assessment proteins present in situ on the latex glove was approximated by staining with Naphthalene Black.
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Abstract
Aspects of protein purification/concentration and detection were modified to enhance sensitivity rapidity and practicality in assaying total proteins from latex gloves. In purifying the eluted proteins by acid precipitation a combination of 5 trichloroacetic acid and 0.2 phosphotungstic acid was found to precipitate proteins most effectively. Maximal colour development in the Lowry micro-assay for proteins was attained when the protein precipitate was re-dissolved in 0.25M sodium hydroxide and when 6 Folin reagent was utilised in the reaction. As a simpler alternative to the colorimetric assay precipitated proteins were quantitated by UV absorption at 280 nm. The UV readings correlated well with results obtained by the Lowry assay. In another approach towards rapid assessment proteins present in situ on the latex glove was approximated by staining with Naphthalene Black.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Rubber industry |
| AGROVOC Term: | Latex |
| AGROVOC Term: | Purification |
| AGROVOC Term: | Precipitation |
| AGROVOC Term: | Trichloroacetic acid |
| AGROVOC Term: | Colour |
| AGROVOC Term: | Sodium hydroxide |
| AGROVOC Term: | Chemical reagents |
| AGROVOC Term: | Absorbance |
| AGROVOC Term: | Spectrophotometers |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 03:58 |
| URI: | http://webagris.upm.edu.my/id/eprint/23406 |
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