Citation
Cardosa M. J., . and Sharifah Hamid, . and Sunderasan E., . and Yeang H. Y., . B-serum is highly immunogenic when compared to C-serum using enzyme immunoassays. pp. 205-211. ISSN 0127-7065
Abstract
Two enzyme immunoassays were utilized to identify the source of antigens eluted from natural rubber latex gloves. Both the indirect enzyme-linked immunosorbent assay (ELISA) and a nitrocellulose based dot enzyme immunoassay showed that a rabbit antiserum directed against proteins eluted from gloves was more reactive against B-serum than against C- serum. It was further shown that the dose response relationship of C-serum and rubber particle extract to rabbit anti-glove protein was similar. When rats were immunised with C-serum an indirect ELISA showed that this serum was more reactive with B-serum antigens than with C-serum suggesting that B-serum is likely to be more immunogenic than C-serum.
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Abstract
Two enzyme immunoassays were utilized to identify the source of antigens eluted from natural rubber latex gloves. Both the indirect enzyme-linked immunosorbent assay (ELISA) and a nitrocellulose based dot enzyme immunoassay showed that a rabbit antiserum directed against proteins eluted from gloves was more reactive against B-serum than against C- serum. It was further shown that the dose response relationship of C-serum and rubber particle extract to rabbit anti-glove protein was similar. When rats were immunised with C-serum an indirect ELISA showed that this serum was more reactive with B-serum antigens than with C-serum suggesting that B-serum is likely to be more immunogenic than C-serum.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Antigens |
| AGROVOC Term: | Natural rubber |
| AGROVOC Term: | Latex |
| AGROVOC Term: | Enzyme linked immunosorbent assay |
| AGROVOC Term: | ELISA |
| AGROVOC Term: | Nitrocellulose |
| AGROVOC Term: | Enzymes |
| AGROVOC Term: | Antiserum |
| AGROVOC Term: | Hypersensitivity |
| AGROVOC Term: | Allergens |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 04:31 |
| URI: | http://webagris.upm.edu.my/id/eprint/23418 |
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