Citation
Shanmugan B., . and Muniandy S., . Purification and properties of glutathione S-transferase from latex of Hevea brasiliensis. pp. 114-123. ISSN 0127-7065
Abstract
Glutathione S-transferase was purified more than 500-fold with a recovery of 50 from the latex of Hevea brasiliensis. The purification steps involved affinity chromatography ion-exchange chromatography and gel filtration. The molecular weight of the native enzyme was 50 000 Daltons as determined by gel filtration while sodium dodecyl sulphate-polyacrylamide gel electrophoresis revealed the presence of one type of sub-unit with a molecular weight of 23 000 Daltons. Substrate specificity studies revealed the enzyme to have limited specificity. The N-terminal amino acid was determined to be glycine while the isoelectric point of this enzyme was estimated to be 4.3. The enzyme exhibited multiplicity on ion-exchange chromatography which could be abolished if the column was elated with thiol reagents.
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Abstract
Glutathione S-transferase was purified more than 500-fold with a recovery of 50 from the latex of Hevea brasiliensis. The purification steps involved affinity chromatography ion-exchange chromatography and gel filtration. The molecular weight of the native enzyme was 50 000 Daltons as determined by gel filtration while sodium dodecyl sulphate-polyacrylamide gel electrophoresis revealed the presence of one type of sub-unit with a molecular weight of 23 000 Daltons. Substrate specificity studies revealed the enzyme to have limited specificity. The N-terminal amino acid was determined to be glycine while the isoelectric point of this enzyme was estimated to be 4.3. The enzyme exhibited multiplicity on ion-exchange chromatography which could be abolished if the column was elated with thiol reagents.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Hevea brasiliensis |
| AGROVOC Term: | Latex |
| AGROVOC Term: | Glutathione |
| AGROVOC Term: | Purification |
| AGROVOC Term: | Transferases |
| AGROVOC Term: | Chromatography |
| AGROVOC Term: | affinity groups |
| AGROVOC Term: | Gel filtration chromatography |
| AGROVOC Term: | Molecular weight |
| AGROVOC Term: | Gel electrophoresis |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:28 |
| URI: | http://webagris.upm.edu.my/id/eprint/23436 |
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