Citation
Cheong K. F., . and Koh C. L., . Nucleotide sequence of the chloroplast gene for the large sub-unit of ribulose-1 5-bisphosphate carboxylase/oxygenase from Hevea brasiliensis. pp. 146-153. ISSN 0127-7065
Abstract
A shotgun genomic library of the chloroplast DNA (ctDNA) of Hevea brasiliensis (clone RRIM 600) was constructed with the plasmid cloning vector pUCl 9 in Escherichia coli. The gene rbcL for the large sub-unit (LS) of ribulose-l 5-bisphosphate carboxylase/oxygenase (Rubisco) was isolated by first screening the library by colony hybridisation and then confirmation by Southern hybridisation with a radiolabelled DNA probe that contains the internal sequence of rbcL from tobacco Nicotiana tabacum. Sequence data indicated that the Hevea rbcL gene contains an open reading frame of 1425 nucleotides encoding a protein of 475 amino acid residues. Conserved sequences in the gene and LS protein were detected. Palindromic sequences which may form intrastrand stem-loop structures for the termination of transcription were encountered at the 3 non-coding region of the gene.
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Abstract
A shotgun genomic library of the chloroplast DNA (ctDNA) of Hevea brasiliensis (clone RRIM 600) was constructed with the plasmid cloning vector pUCl 9 in Escherichia coli. The gene rbcL for the large sub-unit (LS) of ribulose-l 5-bisphosphate carboxylase/oxygenase (Rubisco) was isolated by first screening the library by colony hybridisation and then confirmation by Southern hybridisation with a radiolabelled DNA probe that contains the internal sequence of rbcL from tobacco Nicotiana tabacum. Sequence data indicated that the Hevea rbcL gene contains an open reading frame of 1425 nucleotides encoding a protein of 475 amino acid residues. Conserved sequences in the gene and LS protein were detected. Palindromic sequences which may form intrastrand stem-loop structures for the termination of transcription were encountered at the 3 non-coding region of the gene.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Hevea brasiliensis |
| AGROVOC Term: | Nucleotide sequence |
| AGROVOC Term: | Chloroplasts |
| AGROVOC Term: | Carboxylases |
| AGROVOC Term: | Ribulose-1 5-diphosphate oxygenase |
| AGROVOC Term: | genomics |
| AGROVOC Term: | Chloroplasts |
| AGROVOC Term: | DNA |
| AGROVOC Term: | Clones |
| AGROVOC Term: | Plasmids |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 04:32 |
| URI: | http://webagris.upm.edu.my/id/eprint/23439 |
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