Latex allergy studies: location of soluble proteins in latex examination gloves

Shamsul Bahri A. R., . and Samsidar Hamzah, . and Hafsah Mohd Ghazaly, . and Yeang H. Y., . Latex allergy studies: location of soluble proteins in latex examination gloves. pp. 299-307. ISSN 0127-7065

The cause of soluble proteins eluting mainly from the inner surface of latex gloves as compared with the outer surface was investigated. This differential protein elation from the glove surfaces did not arise from the latex coagulant or cornstarch used in glove manufacture. Eleven commercial brands of latex examination gloves showed greater uptake of the protein stain. Naphthalene Black on the inner glove surface than on the outer surface reflecting the trend in the amounts of proteins extractable from the two surfaces. Light microscope examination of the cross section of the gloves showed a distinct region of soluble proteins stained at the inner surface. The staining was much reduced in samples where the proteins had been earlier removed by leaching with water. Abrading 2 mm (3 of the glove thickness) from the inner glove surface resulted in a 54 decrease of extractable soluble proteins. These results suggested that differential leaching of proteins from the two glove surfaces was not due to the existence of a barrier to protein diffusion. It was best explained by the migration of proteins to the inner glove surface during manufacture.

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Abstract

The cause of soluble proteins eluting mainly from the inner surface of latex gloves as compared with the outer surface was investigated. This differential protein elation from the glove surfaces did not arise from the latex coagulant or cornstarch used in glove manufacture. Eleven commercial brands of latex examination gloves showed greater uptake of the protein stain. Naphthalene Black on the inner glove surface than on the outer surface reflecting the trend in the amounts of proteins extractable from the two surfaces. Light microscope examination of the cross section of the gloves showed a distinct region of soluble proteins stained at the inner surface. The staining was much reduced in samples where the proteins had been earlier removed by leaching with water. Abrading 2 mm (3 of the glove thickness) from the inner glove surface resulted in a 54 decrease of extractable soluble proteins. These results suggested that differential leaching of proteins from the two glove surfaces was not due to the existence of a barrier to protein diffusion. It was best explained by the migration of proteins to the inner glove surface during manufacture.

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Item Type:	Article
AGROVOC Term:	Latex
AGROVOC Term:	Hevea rubber
AGROVOC Term:	Natural rubber
AGROVOC Term:	Proteins
AGROVOC Term:	Fractionation
AGROVOC Term:	Experimentation
AGROVOC Term:	Surface area
AGROVOC Term:	Thickness
AGROVOC Term:	Rubber industry
AGROVOC Term:	Manufactured products
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 04:32
URI:	http://webagris.upm.edu.my/id/eprint/23446

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