Citation
Arokiaraj P., . and Wan Abdul Rahaman W. Y., . Agrobacterium-mediated transformation of Hevea cells derived from in vitro and in vivo seedling cultures. pp. 55-61. ISSN 0127-7065
Abstract
Tumour tissue was produced on stems of in vitro and in vivo propagated rubber (Hevea brasiliensis) seedlings of PB 5/51 infected with a strain of Agrobacterium tumefaciens 541 (isolated by the Rubber Research Institute of Malaysia in 1971). The tumour tissues exhibited phytohormone-independent growth in tissue culture and produced octopine. In contrast controls inoculated with distilled water on seedlings did not produce crown gall. Octopine-utilising Agrobacterium strain 541 was distinguished from non-utilisers on a solid medium containing octopine as the only added nitrogen source with bromothymolblue (BTB) as the pH indicator. On blue BTB plates this strain formed orange-yellow colonies while those formed by non-utilisers were translucent. This process of cell transformation may facilitate the transfer of genes of economic importance to rubber.
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Abstract
Tumour tissue was produced on stems of in vitro and in vivo propagated rubber (Hevea brasiliensis) seedlings of PB 5/51 infected with a strain of Agrobacterium tumefaciens 541 (isolated by the Rubber Research Institute of Malaysia in 1971). The tumour tissues exhibited phytohormone-independent growth in tissue culture and produced octopine. In contrast controls inoculated with distilled water on seedlings did not produce crown gall. Octopine-utilising Agrobacterium strain 541 was distinguished from non-utilisers on a solid medium containing octopine as the only added nitrogen source with bromothymolblue (BTB) as the pH indicator. On blue BTB plates this strain formed orange-yellow colonies while those formed by non-utilisers were translucent. This process of cell transformation may facilitate the transfer of genes of economic importance to rubber.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Hevea brasiliensis |
| AGROVOC Term: | Hevea |
| AGROVOC Term: | Plantlets (seedlings) |
| AGROVOC Term: | Seedlings |
| AGROVOC Term: | In vitro culture |
| AGROVOC Term: | In vivo experimentation |
| AGROVOC Term: | Seeds |
| AGROVOC Term: | Agrobacterium tumefaciens |
| AGROVOC Term: | Plant tissues |
| AGROVOC Term: | Tissue culture |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 04:42 |
| URI: | http://webagris.upm.edu.my/id/eprint/23472 |
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