Antibacterial properties of the microalgae Chaetoceros calcitrans

Pahila Ida G., . and Ticar Bernadeth F., . and De la Peña Milagros R., . and Seraspe Ebonia B., . and Formacion Minda J., . and Amar Edgar C., . Antibacterial properties of the microalgae Chaetoceros calcitrans. pp. 343-356. ISSN 0116-6514

The antibacterial properties of the microalgae Chaetoceros calcitrans were assessed. Samples of C. calcitrans were first extracted in methanol and then in different organic solvents of increasing polarity n-hexane (n-Hex) dichloromethane (DCM) and ethyl acetate (EA) by liquid-liquid extraction. Solvent extracts were screened for antibacterial activity against four species of bacteria: Gram positive Staphylococcus aureus and Bacillus subtilis; and Gram negative Escherichia coli and Vibrio harveyi with Amoxicillin as positive control N-Hex extract with significantly lower antibacterial activity than Amoxicillin showed significantly higher activity than DCM and EA extracts and least in methanolic extract. High antibacterial activity of n-Hex extract against all the microorganisms indicates that the bioactive components could be non-polar since the activity decreased as the solvent became more polar like methanol and finally lost in aqueous extract. Results also showed that the extracts have a broad spectrum activity. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of all solvent extracts on all microorganisms tested ranged from 125 to 500 g.mL�1. Partial purification and characterisation of the extracts confirmed the antibacterial activity in the non-polar fraction which could be terpenes. The results suggest a good prospect in using C. calcitrans against Vibrio and other bacterial species.

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Abstract

The antibacterial properties of the microalgae Chaetoceros calcitrans were assessed. Samples of C. calcitrans were first extracted in methanol and then in different organic solvents of increasing polarity n-hexane (n-Hex) dichloromethane (DCM) and ethyl acetate (EA) by liquid-liquid extraction. Solvent extracts were screened for antibacterial activity against four species of bacteria: Gram positive Staphylococcus aureus and Bacillus subtilis; and Gram negative Escherichia coli and Vibrio harveyi with Amoxicillin as positive control N-Hex extract with significantly lower antibacterial activity than Amoxicillin showed significantly higher activity than DCM and EA extracts and least in methanolic extract. High antibacterial activity of n-Hex extract against all the microorganisms indicates that the bioactive components could be non-polar since the activity decreased as the solvent became more polar like methanol and finally lost in aqueous extract. Results also showed that the extracts have a broad spectrum activity. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of all solvent extracts on all microorganisms tested ranged from 125 to 500 g.mL�1. Partial purification and characterisation of the extracts confirmed the antibacterial activity in the non-polar fraction which could be terpenes. The results suggest a good prospect in using C. calcitrans against Vibrio and other bacterial species.

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Item Type:	Article
AGROVOC Term:	Antibacterial properties
AGROVOC Term:	Polarity
AGROVOC Term:	Methanol
AGROVOC Term:	Solvents
AGROVOC Term:	Gram positive bacteria
AGROVOC Term:	Staphylococcus aureus
AGROVOC Term:	Bacillus subtilis
AGROVOC Term:	Gram negative bacteria
AGROVOC Term:	Escherichia coli
AGROVOC Term:	Vibrio
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23715

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