Citation
Ofor Chukwuma Okereke, . and Udeh Henry, . Effect of water hardness on fertilisation and hatching success of Clarias gariepinus (Burchell 1822) and Heterobranchus longifilis valenciennes 1840 eggs fertilised with C. gariepinus sperm. pp. 270-277. ISSN 0116-6514
Abstract
The effect of water hardness on fertilisation and hatching of eggs of Heterobranchus longifilis Valenciennes 1840 and Clarias gariepinus (Burchell 1822) was studied. Eggs were obtained from five artificially-induced females of each species. Milt from five male C. gariepinus was suspended in 5 mL physiological saline and mixed with 100 g eggs from a homogenised egg mass from five individuals of each species. Two mL aliquots containing 2944 and 2748 C. gariepinus and H. longifilis eggs respectively were incubated at 27 oC in groups of quadruplicate troughs containing water of 20 80 160 300 mg.L� CaCO3 and control dilution medium of 8 mg.L� CaCO3 total hardness. Fertilisation and hatching rates were compared between species using a t-test. Effect of hardness on the variables was determined using the ANOVA. Water hardness level did not significantly affect fertilisation rate of C. gariepinus eggs (p0.05) while H. longifilis eggs were significantly affected.
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Abstract
The effect of water hardness on fertilisation and hatching of eggs of Heterobranchus longifilis Valenciennes 1840 and Clarias gariepinus (Burchell 1822) was studied. Eggs were obtained from five artificially-induced females of each species. Milt from five male C. gariepinus was suspended in 5 mL physiological saline and mixed with 100 g eggs from a homogenised egg mass from five individuals of each species. Two mL aliquots containing 2944 and 2748 C. gariepinus and H. longifilis eggs respectively were incubated at 27 oC in groups of quadruplicate troughs containing water of 20 80 160 300 mg.L� CaCO3 and control dilution medium of 8 mg.L� CaCO3 total hardness. Fertilisation and hatching rates were compared between species using a t-test. Effect of hardness on the variables was determined using the ANOVA. Water hardness level did not significantly affect fertilisation rate of C. gariepinus eggs (p0.05) while H. longifilis eggs were significantly affected.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Water hardness |
| AGROVOC Term: | Fertilization |
| AGROVOC Term: | Hatching |
| AGROVOC Term: | Clarias gariepinus |
| AGROVOC Term: | Heterobranchus longifilis |
| AGROVOC Term: | Sperm |
| AGROVOC Term: | Eggs |
| AGROVOC Term: | Dissolved oxygen |
| AGROVOC Term: | Water temperature |
| AGROVOC Term: | Clariidae |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:28 |
| URI: | http://webagris.upm.edu.my/id/eprint/23731 |
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