Immunomodulation by DNA vaccination against white spot syndrome virus (WSSV)

Korenaga Hiroki, . and Fall Jean, . and Iizasa Toshiyuki, . and Mekata Tohru, . and Kono Tomoya, . and Itami Toshiaki, . and Takayama Hiroaki, . and Sakai Masahiro, . Immunomodulation by DNA vaccination against white spot syndrome virus (WSSV). pp. 435-446. ISSN 0116-6514

Vaccines (subunit and DNA) targeting major envelope proteins VP19 and/or VP28 of white spot syndrome virus (WSSV) in penaeid shrimp were developed and elicited good protection against white spot disease (WSD). However the immune responses in shrimp after administration of these vaccines are not well understood. In this study we developed a DNA vaccine encoding the VP28 envelope protein in kuruma shrimp (Marsupenaeus japonicus) and confirmed the potentiality of protection against WSSV infection. The efficacy of the DNA vaccine against WSSV infection was confirmed by WSSV artificial challenge at 7 days post vaccination in kuruma shrimp. However the efficacy of the vaccine did not last 30 days post vaccination. The transcript of VP28 gene derived from expression vector in tissues of vaccinated shrimp was analyzed by RT-PCR. The transcript of VP28 gene was detected in various tissues including muscle hemolymph gill intestine stomach heart hepatopancreas and lymphoid organ tested at 1 3 and 7 days post vaccination. Subsequently the expression of innate immune-related genes in intestine and lymphoid organ was analyzed at 1 3 and 7 days post vaccination. The expression of innate immune-related genes such as Rab7 penaeidin lysozyme and crustin was up-regulated upon DNA vaccination. These results suggest that DNA vaccination induces significant protection against WSSV by stimulating innate immune responses in kuruma shrimp.

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Abstract

Vaccines (subunit and DNA) targeting major envelope proteins VP19 and/or VP28 of white spot syndrome virus (WSSV) in penaeid shrimp were developed and elicited good protection against white spot disease (WSD). However the immune responses in shrimp after administration of these vaccines are not well understood. In this study we developed a DNA vaccine encoding the VP28 envelope protein in kuruma shrimp (Marsupenaeus japonicus) and confirmed the potentiality of protection against WSSV infection. The efficacy of the DNA vaccine against WSSV infection was confirmed by WSSV artificial challenge at 7 days post vaccination in kuruma shrimp. However the efficacy of the vaccine did not last 30 days post vaccination. The transcript of VP28 gene derived from expression vector in tissues of vaccinated shrimp was analyzed by RT-PCR. The transcript of VP28 gene was detected in various tissues including muscle hemolymph gill intestine stomach heart hepatopancreas and lymphoid organ tested at 1 3 and 7 days post vaccination. Subsequently the expression of innate immune-related genes in intestine and lymphoid organ was analyzed at 1 3 and 7 days post vaccination. The expression of innate immune-related genes such as Rab7 penaeidin lysozyme and crustin was up-regulated upon DNA vaccination. These results suggest that DNA vaccination induces significant protection against WSSV by stimulating innate immune responses in kuruma shrimp.

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Item Type:	Article
AGROVOC Term:	Vaccines
AGROVOC Term:	Vaccination
AGROVOC Term:	Penaeidae
AGROVOC Term:	Prawns and shrimps
AGROVOC Term:	Immune response
AGROVOC Term:	Infection
AGROVOC Term:	Hemolymph
AGROVOC Term:	Gills
AGROVOC Term:	Intestines
AGROVOC Term:	Hepatopancreas
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23739

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