Citation
Ltu Keduzol, . and Singh V. P., . and Kathiresan S., . and Sharma Bhaskar, . and Das Sanjoy, . and Bhilegaonkar K. N., . Detection of Listeria monocytogenes from freshwater fish prawn and chicken meat by direct nested PCR. pp. 432-442. ISSN 0116-6514
Abstract
Three different methods of processing samples from freshwater fish prawn and chicken meat were compared with nested PCR for the detection of Listeria monocytogenes. Nested PCR failed to detect the organism from freshwater fish prawn and chicken meat when processing was done by boiling lysis method but it could detect the organisms from freshwater fish prawn and chicken meat up to the spiking level of 10�´ 10 and 10� cfu.g respectively while processing of sample was done by phenol extraction without enrichment. However using phenol extraction after enrichment the sensitivity was found higher than without enrichment and it was possible to detect up to the spiking level of 10 10 and 10 cfu.g in case of fish prawn and chicken meat respectively.
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Abstract
Three different methods of processing samples from freshwater fish prawn and chicken meat were compared with nested PCR for the detection of Listeria monocytogenes. Nested PCR failed to detect the organism from freshwater fish prawn and chicken meat when processing was done by boiling lysis method but it could detect the organisms from freshwater fish prawn and chicken meat up to the spiking level of 10�´ 10 and 10� cfu.g respectively while processing of sample was done by phenol extraction without enrichment. However using phenol extraction after enrichment the sensitivity was found higher than without enrichment and it was possible to detect up to the spiking level of 10 10 and 10 cfu.g in case of fish prawn and chicken meat respectively.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Listeria monocytogenes |
| AGROVOC Term: | Freshwater fishes |
| AGROVOC Term: | Freshwater prawns and shrimps |
| AGROVOC Term: | Chicken meat |
| AGROVOC Term: | PCR |
| AGROVOC Term: | Boiling |
| AGROVOC Term: | Organisms |
| AGROVOC Term: | Phenols |
| AGROVOC Term: | Labeo rohita |
| AGROVOC Term: | Macrobrachium rosenbergii |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:28 |
| URI: | http://webagris.upm.edu.my/id/eprint/23757 |
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