Citation
Balasubramani A., . Effect of Norethindrone acetate on masculinization growth and reproduction in zebrafish Danio rerio. pp. 44-59. ISSN 0116-6514
Abstract
To masculinize Danio rerio Norethindrone acetate (NE) was administered through discrete immersion (2 hrs each) from the 18th to the 25th day after hatching (dph) at selected concentrations (100 200 400 800 1 000 and 1 200 gl). The immersion at a concentration of 1 000 gl- ensured 100 masculinization and the highest survival (97 ) after treatment and at the age of puberty. Barring this concentration group the NE acted as a growth suppressant and the magnitude of its suppression was stronger in females. During the 240 day experiment sexual maturity in females was postponed from 120th dph in the control to 270th dph in those treated at 1 200 gl and reduced spawning frequency (15 - 3 times) and cumulative fecundity (1 168 - 111 eggs) by reducing the number of vitellogenic eggs. However in the treated males sperm motility was reduced (105 “ 73 sec); consequently fertilizability of the milt drawn from these males was also reduced from 91 to 78 . Progeny testing showed that the cross between males treated at 800 gl and normal females generated the presumed homogametic males which however could induce the females to spawn fewer eggs than that of the presumed heterogametic males.
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Abstract
To masculinize Danio rerio Norethindrone acetate (NE) was administered through discrete immersion (2 hrs each) from the 18th to the 25th day after hatching (dph) at selected concentrations (100 200 400 800 1 000 and 1 200 gl). The immersion at a concentration of 1 000 gl- ensured 100 masculinization and the highest survival (97 ) after treatment and at the age of puberty. Barring this concentration group the NE acted as a growth suppressant and the magnitude of its suppression was stronger in females. During the 240 day experiment sexual maturity in females was postponed from 120th dph in the control to 270th dph in those treated at 1 200 gl and reduced spawning frequency (15 - 3 times) and cumulative fecundity (1 168 - 111 eggs) by reducing the number of vitellogenic eggs. However in the treated males sperm motility was reduced (105 “ 73 sec); consequently fertilizability of the milt drawn from these males was also reduced from 91 to 78 . Progeny testing showed that the cross between males treated at 800 gl and normal females generated the presumed homogametic males which however could induce the females to spawn fewer eggs than that of the presumed heterogametic males.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Danio rerio |
| AGROVOC Term: | Reproduction |
| AGROVOC Term: | Sexual maturity |
| AGROVOC Term: | Spawning |
| AGROVOC Term: | Fecundity |
| AGROVOC Term: | Sperm |
| AGROVOC Term: | Motility |
| AGROVOC Term: | Aquaculture |
| AGROVOC Term: | Androgens |
| AGROVOC Term: | Steroids |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:28 |
| URI: | http://webagris.upm.edu.my/id/eprint/23780 |
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