Study of phenol oxidases in fruits and vegetables: Co-occurrence of thermostable peroxidases and alkaline tyrosinase in a novel source Coccinia grandis.


Citation

Shetty P., . and D’Souza A., . Study of phenol oxidases in fruits and vegetables: Co-occurrence of thermostable peroxidases and alkaline tyrosinase in a novel source Coccinia grandis. pp. 1422-1428. ISSN 2231-7546

Abstract

Tyrosinase and peroxidase content in various fruits and vegetables was compared. Avocado pulp was found to be a rich source of tyrosinase yielding around 4.5U/g. Avocado seeds Datura metel and Coccinia grandis were found to be the next best sources. The tyrosinase rich sources gave comparatively poor yield of peroxidase with the exception of C. grandis. Tyrosinases from Avacado (ATy) functioned optimally at pH 6.5 and was stable over a broad range of pH from 5.5-9. C. grandis tyrosinase (CTy) was an alkaline tyrosinase with an optimum pH of 9.5-10. Optimum temperature of C grandis peroxidase (CPox) was 50 C. ATy CTy and CPox retained 100 activity at 40 C 60 C and 75 C respectively for 30min. The KM for Catechol was 3.2-3.3 mM for both CTy and ATy. KM for Dopa was 3.3 and 1.27mM for ATy and CTy respectively. CPox exhibited a strong affinity for hydrogen peroxide with a KM of 87 M.


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Abstract

Tyrosinase and peroxidase content in various fruits and vegetables was compared. Avocado pulp was found to be a rich source of tyrosinase yielding around 4.5U/g. Avocado seeds Datura metel and Coccinia grandis were found to be the next best sources. The tyrosinase rich sources gave comparatively poor yield of peroxidase with the exception of C. grandis. Tyrosinases from Avacado (ATy) functioned optimally at pH 6.5 and was stable over a broad range of pH from 5.5-9. C. grandis tyrosinase (CTy) was an alkaline tyrosinase with an optimum pH of 9.5-10. Optimum temperature of C grandis peroxidase (CPox) was 50 C. ATy CTy and CPox retained 100 activity at 40 C 60 C and 75 C respectively for 30min. The KM for Catechol was 3.2-3.3 mM for both CTy and ATy. KM for Dopa was 3.3 and 1.27mM for ATy and CTy respectively. CPox exhibited a strong affinity for hydrogen peroxide with a KM of 87 M.

Additional Metadata

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Item Type: Article
AGROVOC Term: Peroxidases
AGROVOC Term: Tyrosinases
AGROVOC Term: Phenols
AGROVOC Term: Fruits
AGROVOC Term: Vegetables
AGROVOC Term: Avocados
AGROVOC Term: Datura metel
AGROVOC Term: affinity groups
AGROVOC Term: Phenolic compounds
AGROVOC Term: Brassica oleracea gongylodes
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:28
URI: http://webagris.upm.edu.my/id/eprint/23788

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