Enzyme aided extraction of sulfated polysaccharides from Turbinaria turbinata brown seaweed

Alam Z., . and Jaswir I., . and Amid A., . and Hammed A. M., . and Simsek S., . Enzyme aided extraction of sulfated polysaccharides from Turbinaria turbinata brown seaweed. pp. 1660-1666. ISSN 2231-7546

This study involves extraction of sulfated polysaccahride (SP) from brown seaweed (Turbinaria turbinata). Eight processing conditions affecting enzyme aided extraction (EAE) were screened using Plackett-Burman design. Three significant factors (hydrolysis time enzyme concentration and extraction stage) were optimized using Faced Centred Central Composite Design in Random Surface Methods. Micrograph obtained using Field Emission Scanning Electron Microscopy revealed that cellulase degradation ruptured the seaweed cell matrix thus caused increase in the release of SP. The optimum conditions for extraction of SP from T. turbinata are: extraction stage of 2 hydrolysis time of 19.5 h and enzyme concentration of 1.5 l/ml to produce 25.13 yield. The SP obtained from cellulase treated T. turbinata is a suitable anti-inflammatory agent for pharmaceutical applications.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(04)%202017/(42).p...

Abstract

This study involves extraction of sulfated polysaccahride (SP) from brown seaweed (Turbinaria turbinata). Eight processing conditions affecting enzyme aided extraction (EAE) were screened using Plackett-Burman design. Three significant factors (hydrolysis time enzyme concentration and extraction stage) were optimized using Faced Centred Central Composite Design in Random Surface Methods. Micrograph obtained using Field Emission Scanning Electron Microscopy revealed that cellulase degradation ruptured the seaweed cell matrix thus caused increase in the release of SP. The optimum conditions for extraction of SP from T. turbinata are: extraction stage of 2 hydrolysis time of 19.5 h and enzyme concentration of 1.5 l/ml to produce 25.13 yield. The SP obtained from cellulase treated T. turbinata is a suitable anti-inflammatory agent for pharmaceutical applications.

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Item Type:	Article
AGROVOC Term:	Brown seaweeds
AGROVOC Term:	Enzymes
AGROVOC Term:	Polysaccharides
AGROVOC Term:	Enzymatic hydrolysis
AGROVOC Term:	Extraction
AGROVOC Term:	Scanning microscopy
AGROVOC Term:	Antiinflammatory agents
AGROVOC Term:	Pharmaceutical industry
AGROVOC Term:	Boiling
AGROVOC Term:	Centrifugation
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23799

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