Citation
Alam Z., . and Jaswir I., . and Amid A., . and Hammed A. M., . and Simsek S., . Enzyme aided extraction of sulfated polysaccharides from Turbinaria turbinata brown seaweed. pp. 1660-1666. ISSN 2231-7546
Abstract
This study involves extraction of sulfated polysaccahride (SP) from brown seaweed (Turbinaria turbinata). Eight processing conditions affecting enzyme aided extraction (EAE) were screened using Plackett-Burman design. Three significant factors (hydrolysis time enzyme concentration and extraction stage) were optimized using Faced Centred Central Composite Design in Random Surface Methods. Micrograph obtained using Field Emission Scanning Electron Microscopy revealed that cellulase degradation ruptured the seaweed cell matrix thus caused increase in the release of SP. The optimum conditions for extraction of SP from T. turbinata are: extraction stage of 2 hydrolysis time of 19.5 h and enzyme concentration of 1.5 l/ml to produce 25.13 yield. The SP obtained from cellulase treated T. turbinata is a suitable anti-inflammatory agent for pharmaceutical applications.
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Abstract
This study involves extraction of sulfated polysaccahride (SP) from brown seaweed (Turbinaria turbinata). Eight processing conditions affecting enzyme aided extraction (EAE) were screened using Plackett-Burman design. Three significant factors (hydrolysis time enzyme concentration and extraction stage) were optimized using Faced Centred Central Composite Design in Random Surface Methods. Micrograph obtained using Field Emission Scanning Electron Microscopy revealed that cellulase degradation ruptured the seaweed cell matrix thus caused increase in the release of SP. The optimum conditions for extraction of SP from T. turbinata are: extraction stage of 2 hydrolysis time of 19.5 h and enzyme concentration of 1.5 l/ml to produce 25.13 yield. The SP obtained from cellulase treated T. turbinata is a suitable anti-inflammatory agent for pharmaceutical applications.
Additional Metadata
Item Type: | Article |
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AGROVOC Term: | Brown seaweeds |
AGROVOC Term: | Enzymes |
AGROVOC Term: | Polysaccharides |
AGROVOC Term: | Enzymatic hydrolysis |
AGROVOC Term: | Extraction |
AGROVOC Term: | Scanning microscopy |
AGROVOC Term: | Antiinflammatory agents |
AGROVOC Term: | Pharmaceutical industry |
AGROVOC Term: | Boiling |
AGROVOC Term: | Centrifugation |
Depositing User: | Ms. Suzila Mohamad Kasim |
Last Modified: | 24 Apr 2025 06:28 |
URI: | http://webagris.upm.edu.my/id/eprint/23799 |
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