Optimization of the enzymatic hydrolysis conditions of waste from shortfin scad (Decapterus Macrosoma) for the production of angiotensin I-converting enzyme (ACE) inhibitory peptide using response surface methodology

Nabilah Husna Ishak, . and Norizah Mhd. Sarbon, . Optimization of the enzymatic hydrolysis conditions of waste from shortfin scad (Decapterus Macrosoma) for the production of angiotensin I-converting enzyme (ACE) inhibitory peptide using response surface methodology. pp. 1735-1743. ISSN 2231-7546

This study aims to optimize enzymatic hydrolysis process for producing angiotensin I-converting enzyme (ACE) inhibitory peptides from protein hydrolysate of shortfin scad (Decapterus Macrosoma) waste (SWH). The enzymatic hydrolysis conditions namely the temperature (40 50 60C) time (B: 60 120 180 min) pH (C: 7 8 9) and enzyme substrate concentrations (D: 1 2 3) on yield degree of hydrolysis (DH) and ACE-inhibitory activity were analysed. Responses were optimized using the response surface methodology (RSM) by employing four factors 3-levels and the Central Composite Design (CCD). The optimized conditions were further validated to indicate the validity of the prediction model. The optimal conditions obtained for the hydrolysis conditions were at temperature of 50C time of 60 min pH of 9 and enzyme to substrate concentration of 2.92. The experimental result for yield was lower than the predicted value as generated by RSM. However the degree of hydrolysis of SWH was higher than the predicted value. The ACE inhibitory activity of SWH was 79.34 and showed lower than the predicted value. Therefore the optimized conditions of SWH served as good conditions for the production of bioactive peptide with high ACE inhibitory activity.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(04)%202017/(51).p...

Abstract

This study aims to optimize enzymatic hydrolysis process for producing angiotensin I-converting enzyme (ACE) inhibitory peptides from protein hydrolysate of shortfin scad (Decapterus Macrosoma) waste (SWH). The enzymatic hydrolysis conditions namely the temperature (40 50 60C) time (B: 60 120 180 min) pH (C: 7 8 9) and enzyme substrate concentrations (D: 1 2 3) on yield degree of hydrolysis (DH) and ACE-inhibitory activity were analysed. Responses were optimized using the response surface methodology (RSM) by employing four factors 3-levels and the Central Composite Design (CCD). The optimized conditions were further validated to indicate the validity of the prediction model. The optimal conditions obtained for the hydrolysis conditions were at temperature of 50C time of 60 min pH of 9 and enzyme to substrate concentration of 2.92. The experimental result for yield was lower than the predicted value as generated by RSM. However the degree of hydrolysis of SWH was higher than the predicted value. The ACE inhibitory activity of SWH was 79.34 and showed lower than the predicted value. Therefore the optimized conditions of SWH served as good conditions for the production of bioactive peptide with high ACE inhibitory activity.

[error in script]

Item Type:	Article
AGROVOC Term:	Enzymatic hydrolysis
AGROVOC Term:	Decapterus macrosoma
AGROVOC Term:	Angiotensin
AGROVOC Term:	Enzyme inhibitors
AGROVOC Term:	Peptides
AGROVOC Term:	Protein hydrolysate
AGROVOC Term:	Fish wastes
AGROVOC Term:	Temperature
AGROVOC Term:	Time
AGROVOC Term:	Fish processing
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 05:28
URI:	http://webagris.upm.edu.my/id/eprint/23807

Actions (login required)

View Item