Identification of Escherichia spp. strains in street-vended beverages and associated preparation surfaces using 16S rRNA analysis

Chong S. Y., . and Rao P. V., . and Soon J. M., . Identification of Escherichia spp. strains in street-vended beverages and associated preparation surfaces using 16S rRNA analysis. pp. 1811-1818. ISSN 2231-7546

Street-vended beverages are commonly prepared by mechanical extraction of the pulp usually unpasteurised and requires multiple handling steps. Foodborne pathogens transmitted via skin of street vendors or via faecal-oral route may contaminate the preparation surfaces and beverages. The aim of this study is to identify Escherichia spp. strains of street-vended beverages and their associated preparation surfaces using 16s rRNA analysis. The hygienic practice of vendors was represented by Staphylococcus spp. analysis and Staphylococcus aureus is not detected in beverages and associated preparation surfaces. A total of 80 samples (18 beverages 15 swab samples and 47 direct film samples) were collected followed by enumeration of microbial load. Polymerase Chain Reaction (PCR) amplification and 16S ribosomal ribonucleic acid (rRNA) sequencing were carried out. Results of 16S rRNA sequence analysis indicated that three gramnegative isolates were identified as Escherichia coli RM9387 (Accession no. CP009104.1) Escherichia coli c164 (Accession no. JQ781646.1) and Escherichia fergusonii E10 (Accession no. KJ626264.1) with similarity value of 99 respectively.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(04)%202017/(60).p...

Abstract

Street-vended beverages are commonly prepared by mechanical extraction of the pulp usually unpasteurised and requires multiple handling steps. Foodborne pathogens transmitted via skin of street vendors or via faecal-oral route may contaminate the preparation surfaces and beverages. The aim of this study is to identify Escherichia spp. strains of street-vended beverages and their associated preparation surfaces using 16s rRNA analysis. The hygienic practice of vendors was represented by Staphylococcus spp. analysis and Staphylococcus aureus is not detected in beverages and associated preparation surfaces. A total of 80 samples (18 beverages 15 swab samples and 47 direct film samples) were collected followed by enumeration of microbial load. Polymerase Chain Reaction (PCR) amplification and 16S ribosomal ribonucleic acid (rRNA) sequencing were carried out. Results of 16S rRNA sequence analysis indicated that three gramnegative isolates were identified as Escherichia coli RM9387 (Accession no. CP009104.1) Escherichia coli c164 (Accession no. JQ781646.1) and Escherichia fergusonii E10 (Accession no. KJ626264.1) with similarity value of 99 respectively.

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Item Type:	Article
AGROVOC Term:	Escherichia
AGROVOC Term:	Identification
AGROVOC Term:	Beverages
AGROVOC Term:	RNA
AGROVOC Term:	Extraction
AGROVOC Term:	Pulp
AGROVOC Term:	Pasteurization
AGROVOC Term:	Food hygiene
AGROVOC Term:	Staphylococcus
AGROVOC Term:	Escherichia coli
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23816

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