Effect of protein fractions from Pithecellobium jiringa on secretions of interleukin-6 and leptin in 3T3-L1 preadipocytes

Yanti, . and Woenardhy K., . and Widjaja A. Y., . and Agustinah W., . Effect of protein fractions from Pithecellobium jiringa on secretions of interleukin-6 and leptin in 3T3-L1 preadipocytes. pp. 2146-2152. ISSN 2231-7546

Obesity is a prevalent metabolic disorder that is induced by inflammatory responses that lead to the increased levels of pro-inflammatory mediators including interleukin-6 (IL-6) and leptin. Pithecellobium jiringa or jengkol is a functional tropical plant in Southeast Asia regions including Indonesia that has been for culinary and traditional medicines. This research was aimed to identify chemical constituents of protein fractions from P. jiringa peel and seed by using pyrolysis gas chromatography mass spectrometry (py-GC/MS) and their inhibitory effects at various concentrations (5-100 g/ml) on IL-6 and leptin expression in mouse 3T3-L1 preadipocytes by conducting the enzyme-linked immunosorbent assay (ELISA). Both protein fractions from P. jiringa peel and seed consisted of amino acid derivatives including alanine ethyl ester (peel part) dimehylamine piperidine aziridine and phenyl acetyl glutamine (seed part). Protein fractions from P. jiringa peel at 25 g/ml decreased 50 leptin expression in 3T3-L1 preadipocytes compared to that of seed protein fractions. Meanwhile seed protein fraction dose-dependently inhibited IL-6 expression. At 10 g/ml seed protein fraction reduced up to 50 IL-6 production respectively. Our results suggest that protein fractions from both P. jiringa peel and seed may have potential anti-obesity effects on 3T3-L1 preadipocytes via attenuating IL-6 and leptin expression. P. jiringa protein fractions can be used as alternative food supplements for reducing the risk of obesity.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(05)%202017/(43).p...

Abstract

Obesity is a prevalent metabolic disorder that is induced by inflammatory responses that lead to the increased levels of pro-inflammatory mediators including interleukin-6 (IL-6) and leptin. Pithecellobium jiringa or jengkol is a functional tropical plant in Southeast Asia regions including Indonesia that has been for culinary and traditional medicines. This research was aimed to identify chemical constituents of protein fractions from P. jiringa peel and seed by using pyrolysis gas chromatography mass spectrometry (py-GC/MS) and their inhibitory effects at various concentrations (5-100 g/ml) on IL-6 and leptin expression in mouse 3T3-L1 preadipocytes by conducting the enzyme-linked immunosorbent assay (ELISA). Both protein fractions from P. jiringa peel and seed consisted of amino acid derivatives including alanine ethyl ester (peel part) dimehylamine piperidine aziridine and phenyl acetyl glutamine (seed part). Protein fractions from P. jiringa peel at 25 g/ml decreased 50 leptin expression in 3T3-L1 preadipocytes compared to that of seed protein fractions. Meanwhile seed protein fraction dose-dependently inhibited IL-6 expression. At 10 g/ml seed protein fraction reduced up to 50 IL-6 production respectively. Our results suggest that protein fractions from both P. jiringa peel and seed may have potential anti-obesity effects on 3T3-L1 preadipocytes via attenuating IL-6 and leptin expression. P. jiringa protein fractions can be used as alternative food supplements for reducing the risk of obesity.

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Item Type:	Article
AGROVOC Term:	Pithecellobium
AGROVOC Term:	Metabolic disorders
AGROVOC Term:	Interleukins
AGROVOC Term:	Leptin
AGROVOC Term:	Extraction
AGROVOC Term:	Enzyme linked immunosorbent assay
AGROVOC Term:	Gas chromatography
AGROVOC Term:	Mass spectrometry
AGROVOC Term:	Inhibition
AGROVOC Term:	Cell culture
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23856

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