Assessment of biosynthesis of silver nanoparticles from Punica granum (peels and arils)

Razifah Mohd Razali, . and Siti Aliyah Mohd Ismail, . and Fatimah Hashim, . Assessment of biosynthesis of silver nanoparticles from Punica granum (peels and arils). pp. 1-16. ISSN 2180-1983

In this study we designed a simple and eco-friendly biosynthesis of silver nanoparticles from peels and arils extract of Punica granatum (pomegranate) which act as a reducing agent in AgNO3 solution. The formation of silver nanoparticles was spectroscopically characterized by UV-Vis spectroscopy analysis and results exhibited an absorbance spectrum peak between the range of 282-432 nm within 24 hours when centred on the surface of silver nanoparticles. Further investigation on antioxidant activity via DPPH assay showed higher scavenging effect at 84.06 in AgNPs synthesized from peels extract compared to 80.16 for AgNPs synthesized from arils. Both AgNPs also exhibited antibacterial activity against two species of Gram positive bacteria (Bacillus subtilis Staphylococcus aureus) and two species of Gram negative bacteria (Klebsiella pneumoniae Pseudomonas aeruginosa). These AgNPs were also assayed for their antiamoebic properties towards Acanthamoeba sp. in order to determine their cytotoxicity effect and the IC50 values obtained were 33.08 v/v and 2.33 v/v for peels and arils extracts respectively. These results indicate that both arils and peels of P. granatum have great potential for the synthesis of AgNPs that have antioxidant antibacterial and antiamoebic activities.

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Abstract

In this study we designed a simple and eco-friendly biosynthesis of silver nanoparticles from peels and arils extract of Punica granatum (pomegranate) which act as a reducing agent in AgNO3 solution. The formation of silver nanoparticles was spectroscopically characterized by UV-Vis spectroscopy analysis and results exhibited an absorbance spectrum peak between the range of 282-432 nm within 24 hours when centred on the surface of silver nanoparticles. Further investigation on antioxidant activity via DPPH assay showed higher scavenging effect at 84.06 in AgNPs synthesized from peels extract compared to 80.16 for AgNPs synthesized from arils. Both AgNPs also exhibited antibacterial activity against two species of Gram positive bacteria (Bacillus subtilis Staphylococcus aureus) and two species of Gram negative bacteria (Klebsiella pneumoniae Pseudomonas aeruginosa). These AgNPs were also assayed for their antiamoebic properties towards Acanthamoeba sp. in order to determine their cytotoxicity effect and the IC50 values obtained were 33.08 v/v and 2.33 v/v for peels and arils extracts respectively. These results indicate that both arils and peels of P. granatum have great potential for the synthesis of AgNPs that have antioxidant antibacterial and antiamoebic activities.

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Item Type:	Article
AGROVOC Term:	Punica granatum
AGROVOC Term:	Pomegranates
AGROVOC Term:	Punicaceae
AGROVOC Term:	Biosynthesis
AGROVOC Term:	Antibacterial properties
AGROVOC Term:	Plant resources
AGROVOC Term:	Extraction
AGROVOC Term:	Peel
AGROVOC Term:	Centrifugation
AGROVOC Term:	Spectroscopy
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23896

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