Analysis of beef meatball adulteration with wild boar meat using real-time polymerase chain reaction

Guntarti A., . and Martono S., . and Yuswanto A., . and Rohman A., . Analysis of beef meatball adulteration with wild boar meat using real-time polymerase chain reaction. pp. 2451-2455. ISSN 2231-7546

The price of beef is high in Indonesia so that some unethical producers try to blend beef with wild boar meat (WBM) in meatball formulation to gain economic profits. This study aims to use real-time polymerase chain reaction (RT-PCR) for identification of WBA in meatball formulation. The specific primers used are designed using NCBI software and subjected to BLAST procedure. The candidate primers are tested for specificity study using several DNAs from fresh meat of some species including pork having similar characteristics with WBM. The developed method is also validated by determining several parameters of linearity sensitivity precision and efficiency. The results showed that primer CytbAG3A at specific annealing temperature can amplify specifically DNA from WBM with acceptable efficiency (105). The detection limit of WBM DNA is 48 pg corresponding to 3 of WBM in beef meatball formulation. The developed method is used for analysis of marketed meatballs from Yogyakarta (representative for non-wild boar area) Kalimantan (as representative region of wild boar area) and Timor Leste (representative of other country and wild boar area). RT-PCR using CytbAG3A primer is accurate and reliable method for analysis of WBM in meatball in order to support halal authentication of food products.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(06)%202017/(24).p...

Abstract

The price of beef is high in Indonesia so that some unethical producers try to blend beef with wild boar meat (WBM) in meatball formulation to gain economic profits. This study aims to use real-time polymerase chain reaction (RT-PCR) for identification of WBA in meatball formulation. The specific primers used are designed using NCBI software and subjected to BLAST procedure. The candidate primers are tested for specificity study using several DNAs from fresh meat of some species including pork having similar characteristics with WBM. The developed method is also validated by determining several parameters of linearity sensitivity precision and efficiency. The results showed that primer CytbAG3A at specific annealing temperature can amplify specifically DNA from WBM with acceptable efficiency (105). The detection limit of WBM DNA is 48 pg corresponding to 3 of WBM in beef meatball formulation. The developed method is used for analysis of marketed meatballs from Yogyakarta (representative for non-wild boar area) Kalimantan (as representative region of wild boar area) and Timor Leste (representative of other country and wild boar area). RT-PCR using CytbAG3A primer is accurate and reliable method for analysis of WBM in meatball in order to support halal authentication of food products.

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Item Type:	Article
AGROVOC Term:	Wild boar
AGROVOC Term:	Meat
AGROVOC Term:	Pig meat
AGROVOC Term:	Meat products
AGROVOC Term:	Processed foods
AGROVOC Term:	Adulteration
AGROVOC Term:	Isolation
AGROVOC Term:	Identification
AGROVOC Term:	analysis
AGROVOC Term:	DNA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23906

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