Antihypertensive property of the peptic and chymotryptic hydrolysates derived from the crude protein extract of okra Abelmoschus esculentus (L.) Moench seeds

Castillo I. J. B., . and Angelia M. R. N., . and Torio M. A. O., . and Belina-Aldemita M. D., . Antihypertensive property of the peptic and chymotryptic hydrolysates derived from the crude protein extract of okra Abelmoschus esculentus (L.) Moench seeds. pp. 2586-2592. ISSN 2231-7546

Bioactive peptides from okra seeds were successfully isolated purified and characterized for its antihypertensive properties. Okra seeds were decorticated defatted and suspended in 35 mM phosphate buffer (pH 7.6 with 0.4 M NaCl and 0.02 NaN) to isolate the okra seed crude protein extract (OSCPE). The protein content of OSCPE was estimated to be 13.8 0.6 mg/mL. Sodium dodecyl sulfate-polyacrylamide-gel electrophoresis (SDS-PAGE) revealed six major polypeptide bands with molecular weights of 11 13 16 18 25 and 49 kDa.Bioactive peptides from OSCPE were released through separate enzymatic hydrolysis using pepsin and -chymotrypsin from 0 min to 24 h of incubation. OSCPE was more resistant to enzymatic hydrolysis with -chymotrypsin than pepsin. The 12-h hydrolysate from both enzymes exhibited the highest angiotensin I-converting enzyme (ACE) inhibitory activity. Both hydrolysates were fractionated through anion-exchange chromatography (IEX) using a DEAE-Sephacel column (1.6 cm 5.0 cm). Fractions from prominent peaks were subjected to ACE inhibition assay. The 17th peptic IEX fraction and the 15th chymotryptic IEX fraction showed the highest ACE inhibitory activities of 68.68 2.91 and 71.89 7.77 with IC50 values of 8.62 1.17 g/mL and 2.44 0.87 g/mL respectively. Aside from sustaining an individuals daily protein requirements okra seeds also have the potential to further improve health by releasing bioactive peptides that can alleviate hypertension.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(06)%202017/(42).p...

Abstract

Bioactive peptides from okra seeds were successfully isolated purified and characterized for its antihypertensive properties. Okra seeds were decorticated defatted and suspended in 35 mM phosphate buffer (pH 7.6 with 0.4 M NaCl and 0.02 NaN) to isolate the okra seed crude protein extract (OSCPE). The protein content of OSCPE was estimated to be 13.8 0.6 mg/mL. Sodium dodecyl sulfate-polyacrylamide-gel electrophoresis (SDS-PAGE) revealed six major polypeptide bands with molecular weights of 11 13 16 18 25 and 49 kDa.Bioactive peptides from OSCPE were released through separate enzymatic hydrolysis using pepsin and -chymotrypsin from 0 min to 24 h of incubation. OSCPE was more resistant to enzymatic hydrolysis with -chymotrypsin than pepsin. The 12-h hydrolysate from both enzymes exhibited the highest angiotensin I-converting enzyme (ACE) inhibitory activity. Both hydrolysates were fractionated through anion-exchange chromatography (IEX) using a DEAE-Sephacel column (1.6 cm 5.0 cm). Fractions from prominent peaks were subjected to ACE inhibition assay. The 17th peptic IEX fraction and the 15th chymotryptic IEX fraction showed the highest ACE inhibitory activities of 68.68 2.91 and 71.89 7.77 with IC50 values of 8.62 1.17 g/mL and 2.44 0.87 g/mL respectively. Aside from sustaining an individuals daily protein requirements okra seeds also have the potential to further improve health by releasing bioactive peptides that can alleviate hypertension.

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Item Type:	Article
AGROVOC Term:	Abelmoschus esculentus
AGROVOC Term:	Okras
AGROVOC Term:	Seed
AGROVOC Term:	Crude protein
AGROVOC Term:	Isolation
AGROVOC Term:	Extraction
AGROVOC Term:	Hydrolysis
AGROVOC Term:	In vitro
AGROVOC Term:	Protein hydrolysate
AGROVOC Term:	Enzymatic hydrolysis
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23924

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