Identification of methicillin-resistant staphylococci isolated from milk and milk products of Southern Assam India and evaluation of their enterotoxigenicity

Mazumder P. B., . and Mohanta A., . and Bhattacharjee A., . Identification of methicillin-resistant staphylococci isolated from milk and milk products of Southern Assam India and evaluation of their enterotoxigenicity. pp. 2705-2710. ISSN 2231-7546

In this study methicillin-resistant staphylococci isolated from milk and milk products were evaluated for their enterotoxigenic potential with emphasis on the three classical enterotoxin genes namely SEA SEB and SEC. 11(35.48) out of 31 staphylococcal isolates were found to be methicillin-resistant being positive for mecA gene. Of these 11 methicillin-resistant isolates 8 (72.73) were positive for either SEA or SEC. Sequencing of the 16S rDNA helped identify the methicillin-resistant isolates as S. aureus S. sciuri S. epidermidis and S. saprophyticus. Phylogenetic tree constructed with the 16S rDNA sequences revealed a close relationship among the isolates that accounts for dissemination of genes like those for antibiotic resistance and enterotoxins.The presence of methicillin-resistant enterotoxigenic strains of Staphylococcus in popular food products like milk and milk products analysed in this study indicates potential health risk for consumers and emphasizes the necessity of hygienic handling of these food products.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(06)%202017/(59).p...

Abstract

In this study methicillin-resistant staphylococci isolated from milk and milk products were evaluated for their enterotoxigenic potential with emphasis on the three classical enterotoxin genes namely SEA SEB and SEC. 11(35.48) out of 31 staphylococcal isolates were found to be methicillin-resistant being positive for mecA gene. Of these 11 methicillin-resistant isolates 8 (72.73) were positive for either SEA or SEC. Sequencing of the 16S rDNA helped identify the methicillin-resistant isolates as S. aureus S. sciuri S. epidermidis and S. saprophyticus. Phylogenetic tree constructed with the 16S rDNA sequences revealed a close relationship among the isolates that accounts for dissemination of genes like those for antibiotic resistance and enterotoxins.The presence of methicillin-resistant enterotoxigenic strains of Staphylococcus in popular food products like milk and milk products analysed in this study indicates potential health risk for consumers and emphasizes the necessity of hygienic handling of these food products.

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Item Type:	Article
AGROVOC Term:	Staphylococcus
AGROVOC Term:	Staphylococcus aureus
AGROVOC Term:	Staphylococcus epidermidis
AGROVOC Term:	Milk
AGROVOC Term:	Enterotoxins
AGROVOC Term:	Antibiotics
AGROVOC Term:	Drug resistance
AGROVOC Term:	Evaluation
AGROVOC Term:	Identification
AGROVOC Term:	Isolation
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23938

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