Characteristics for ornithine-production and ornithine-synthase from Bifidobacterium longum


Citation

Hahn B. S., . and Surh J., . and Kang S. A., . and Choi K. H., . and Chung H. Y., . and Jang K. H., . Characteristics for ornithine-production and ornithine-synthase from Bifidobacterium longum. pp. 2711-2715. ISSN 2231-7546

Abstract

Bifidobacterium longum KCTC 5734 was evaluated for use in sunsik fermentation where sunsik Korean heated cereal powder was used as the sole growth medium. Interestingly B. longum KCTC 5734 was found to produce ornithine (12.3 ppm) from sunsik. In order to confirm the presence of an ornithine production pathway in this strain genomic DNA was purified and amplified using PCR products were cloned into Escherichia coli and the amino acids sequence of the vector was used for multiple alignment analysis. The results identified 2 genes in the genome of B. longum KCTC 5734 coding for a putative N-acetylornithine aminotransferase and N-acetylglutamate synthase/ornithine acetyltransferase. This indicates that B. longum KCTC 5734 can be used as a starter culture for the production of ornithine-rich foods.


Download File

Full text available from:

Abstract

Bifidobacterium longum KCTC 5734 was evaluated for use in sunsik fermentation where sunsik Korean heated cereal powder was used as the sole growth medium. Interestingly B. longum KCTC 5734 was found to produce ornithine (12.3 ppm) from sunsik. In order to confirm the presence of an ornithine production pathway in this strain genomic DNA was purified and amplified using PCR products were cloned into Escherichia coli and the amino acids sequence of the vector was used for multiple alignment analysis. The results identified 2 genes in the genome of B. longum KCTC 5734 coding for a putative N-acetylornithine aminotransferase and N-acetylglutamate synthase/ornithine acetyltransferase. This indicates that B. longum KCTC 5734 can be used as a starter culture for the production of ornithine-rich foods.

Additional Metadata

[error in script]
Item Type: Article
AGROVOC Term: Bifidobacterium
AGROVOC Term: Ornithine
AGROVOC Term: Biochemistry
AGROVOC Term: Amino acids
AGROVOC Term: DNA
AGROVOC Term: Enzymes
AGROVOC Term: Fermentation
AGROVOC Term: Foods
AGROVOC Term: Cell culture
AGROVOC Term: Escherichia coli
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:28
URI: http://webagris.upm.edu.my/id/eprint/23939

Actions (login required)

View Item View Item