Citation
Sarina Sulaiman, . and Jamal P., . and Ansari A. H., . and Azlin Suhaida Azmi, . and Nurul Najihah Ilias, . and Zaki Zainudin, . and Jaswir I., . Optimum media compositions for bioprotein production by mixed culture of Phanerochaete chrysosporium and Candida utilis using palm kernel cake and seaweed as substrates. S363-S368. ISSN 2231-7546
Abstract
Bioprotein is one of the useful products obtained from biotechnology invention. It is a promising replacement for the commercial fish feed supplement. In this study the enrichment of the bioprotein content after solid state fermentation using palm kernel cake and seaweed by the white rot fungus: Phanerochaete chrysoporium and yeast: Candida utilis was carried out. The growth media components were selected from 11 types of media using Plackett-Burman design (hereinafter PBD) and were optimized by one-factor-at-a-time (OFAT) method with bioprotein concentration (mg/g) as the response. From the screening result using PBD three media components namely K2HPO4 CuSO4.5H2O and MnSO4.H2O were selected for further optimization using OFAT method because of their positive contributions to the response. The final results showed that 5.0 g/L K2 HPO4 3.0 g/L CuSO4.5H2O and 0.1 g/L MnSO4.H2O werethere to be the optimum media constituents with 9.0 g/L MgSO4.7H2O 0.1 g/L CaCl2.H2O 3.0g/L FeSO4.7H2O and 3.0 g/L peptone as fixed compositions. At this optimum concentration the protein increment of 11 was observed as compared to the results determined in the screening using PBD. The study revealed the benefits of using mixed cultures in improving the protein concentrations which can be used as nutritious fish feed.
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Abstract
Bioprotein is one of the useful products obtained from biotechnology invention. It is a promising replacement for the commercial fish feed supplement. In this study the enrichment of the bioprotein content after solid state fermentation using palm kernel cake and seaweed by the white rot fungus: Phanerochaete chrysoporium and yeast: Candida utilis was carried out. The growth media components were selected from 11 types of media using Plackett-Burman design (hereinafter PBD) and were optimized by one-factor-at-a-time (OFAT) method with bioprotein concentration (mg/g) as the response. From the screening result using PBD three media components namely K2HPO4 CuSO4.5H2O and MnSO4.H2O were selected for further optimization using OFAT method because of their positive contributions to the response. The final results showed that 5.0 g/L K2 HPO4 3.0 g/L CuSO4.5H2O and 0.1 g/L MnSO4.H2O werethere to be the optimum media constituents with 9.0 g/L MgSO4.7H2O 0.1 g/L CaCl2.H2O 3.0g/L FeSO4.7H2O and 3.0 g/L peptone as fixed compositions. At this optimum concentration the protein increment of 11 was observed as compared to the results determined in the screening using PBD. The study revealed the benefits of using mixed cultures in improving the protein concentrations which can be used as nutritious fish feed.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Phanerochaete chrysosporium |
| AGROVOC Term: | Candida utilis |
| AGROVOC Term: | Aquatic plants |
| AGROVOC Term: | Palm kernel cake |
| AGROVOC Term: | Seaweeds |
| AGROVOC Term: | Growth media |
| AGROVOC Term: | Fermentation |
| AGROVOC Term: | Sargassum |
| AGROVOC Term: | Crude protein |
| AGROVOC Term: | Gallic acid |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 05:34 |
| URI: | http://webagris.upm.edu.my/id/eprint/23955 |
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