An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response

Yusof F., . and Faruck M. O., . and Chowdhury S., . An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response. S457-S462. ISSN 2231-7546

Antifungal peptides have been successfully extracted from whole body larvae of Zophobas morio (Fabricius) by using acidified isopropanol. To ensure that the extraction is cost effective for maximum yield Response Surface Methodology (RSM) using a Central Composite Design (CCD) strategy was adopted to optimize the extraction process parameters. The effect of independent parameters namely the homogenization temperature (C) homogenization time (min) and solid (g) to the solvent (ml) ratio of the extraction process on the fungal growth was studied. The extracted samples obtained by conducting runs accorded by the experimental design showed varying degree of antifungal activity against Aspergillus niger the selected fungal strain as assayed by the Poisoned agar technique. The investigation showed that the optimum values of the extraction parameters for the maximum antifungal peptides were 5 minutes homogenization time 4C homogenization temperature and 3.5:1 solid to solvent ratio. This study reports the development of an extraction process that allows careful recovery of antifungal peptides from larvae. In the validation of the experimental model the error between the actual value and the predicted value was determined to be 3.57.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(07)%202017%20supp...

Abstract

Antifungal peptides have been successfully extracted from whole body larvae of Zophobas morio (Fabricius) by using acidified isopropanol. To ensure that the extraction is cost effective for maximum yield Response Surface Methodology (RSM) using a Central Composite Design (CCD) strategy was adopted to optimize the extraction process parameters. The effect of independent parameters namely the homogenization temperature (C) homogenization time (min) and solid (g) to the solvent (ml) ratio of the extraction process on the fungal growth was studied. The extracted samples obtained by conducting runs accorded by the experimental design showed varying degree of antifungal activity against Aspergillus niger the selected fungal strain as assayed by the Poisoned agar technique. The investigation showed that the optimum values of the extraction parameters for the maximum antifungal peptides were 5 minutes homogenization time 4C homogenization temperature and 3.5:1 solid to solvent ratio. This study reports the development of an extraction process that allows careful recovery of antifungal peptides from larvae. In the validation of the experimental model the error between the actual value and the predicted value was determined to be 3.57.

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Item Type:	Article
AGROVOC Term:	Tenebrionidae
AGROVOC Term:	Antifungal properties
AGROVOC Term:	Animal models
AGROVOC Term:	Fungicidal properties
AGROVOC Term:	Extraction
AGROVOC Term:	Homogenization
AGROVOC Term:	Temperature
AGROVOC Term:	Time
AGROVOC Term:	Methodology
AGROVOC Term:	Experimental design
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23967

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