Effect of extraction process parameters on antifungal peptides from Supermeal worm Zophobas morio (Fabricius)

Faruck M. O., . and Yusof F., . and Chowdhury S., . Effect of extraction process parameters on antifungal peptides from Supermeal worm Zophobas morio (Fabricius). S463-S467. ISSN 2231-7546

Acidified isopropanol extract of whole body larvae of Zophobas morio (Fabricius) which contains peptides has been shown to exhibit an inhibitory effect towards fungal growth. The larvae commonly known as supermeal worm are cheap and easily maintained. To make the extraction even more cost effective it is pertinent to maximize the extraction yield and to optimize the extraction process. The aim of this study is to use the One-Factor-At-a-Time (OFAT) strategy to determine the maximum values of the process parameters for the extraction of antifungal peptides where these values can later be used in the experimental design to optimize the extraction process. Based on importance three parameters were selected namely initial homogenization temperature homogenization time and solid (g) to solvent (ml) ratio. Maximum inhibition to fungal growth was found when the extraction was carried out as follows; using initial homogenization temperature of 4C homogenization time of 5 minutes and a solid (g) to solvent (ml) ratio of 3.5:1. The peptide extract displayed different degree of antifungal effect towards four selected fungi Aspergillus niger Microsporum canis Candida albicans and Blastomyces dermatitidsis.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(07)%202017%20supp...

Abstract

Acidified isopropanol extract of whole body larvae of Zophobas morio (Fabricius) which contains peptides has been shown to exhibit an inhibitory effect towards fungal growth. The larvae commonly known as supermeal worm are cheap and easily maintained. To make the extraction even more cost effective it is pertinent to maximize the extraction yield and to optimize the extraction process. The aim of this study is to use the One-Factor-At-a-Time (OFAT) strategy to determine the maximum values of the process parameters for the extraction of antifungal peptides where these values can later be used in the experimental design to optimize the extraction process. Based on importance three parameters were selected namely initial homogenization temperature homogenization time and solid (g) to solvent (ml) ratio. Maximum inhibition to fungal growth was found when the extraction was carried out as follows; using initial homogenization temperature of 4C homogenization time of 5 minutes and a solid (g) to solvent (ml) ratio of 3.5:1. The peptide extract displayed different degree of antifungal effect towards four selected fungi Aspergillus niger Microsporum canis Candida albicans and Blastomyces dermatitidsis.

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Item Type:	Article
AGROVOC Term:	Antifungal properties
AGROVOC Term:	Tenebrionidae
AGROVOC Term:	Aspergillus niger
AGROVOC Term:	Microsporum
AGROVOC Term:	Candida albicans
AGROVOC Term:	Blastomyces
AGROVOC Term:	Extraction
AGROVOC Term:	Extracts
AGROVOC Term:	Homogenization
AGROVOC Term:	Centrifugation
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23968

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