Optimization of essential oil and fucoxanthin extraction from Sargassum binderi by Supercritical Carbon Dioxide (SC-CO) extraction with ethanol as co-solvent Using Response Surface Methodology (RSM)

Jaswir I., . and Fitri Octavianti, . and Alias Abdullah, . and Hasna Ahmad, . and Hamzah Mohd Salleh, . and M. Taher Bakhtiar, . and Noviendri D., . and Miyashita K., . and Lestari W., . and Hendri R., . and Farahidah Mohamed, . Optimization of essential oil and fucoxanthin extraction from Sargassum binderi by Supercritical Carbon Dioxide (SC-CO) extraction with ethanol as co-solvent Using Response Surface Methodology (RSM). S514-S521. ISSN 2231-7546

Supercritical carbon dioxide (SC-CO) extraction of fucoxanthin is more advantageous over conventional solvent extraction as it is less toxic less hazardous to the environment and preserves the bioactivity of fucoxanthin. A face-centered central composite design (FCCCD) based on response surface methodology (RSM) was employed for SC-CO extraction of oils and fucoxanthin from the brown seaweed Sargassum binderi with ethanol as a co- solvent. Three independent parameters namely extraction temperature (A: 40 50 60C) pressure (B:2900 3625 4350 psig and particle size (C: 90 500 and 1000 m) were investigated to optimize extraction oil yields (EOY) and fucoxanthin yields (FY). A regression model was developed tested for quality of fit (R‚‚) and expressed in the form of 3D response surface curve and 2D contour. The optimum extraction conditions were obtained at extraction temperature (A) 50C pressure (B) 3625 psig and particle size (C) 500 m. Under these conditions optimal EOY and FY were 10.04 mg/g and 3188.99 g/g respectively. The difference between the lowest and the highest response in EOY and FY were 5.44 “ 10.04 mg/g and 2109.10 - 3188.90 g/g respectively. The lowest yields were identified at 60C 2900 psig and 1000 m. The regression models generated showing interactions between the variables and EOY and FY response were significant as tested by ANOVA (p 0.0005 and p 0.0007 respectively) with high R‚‚ values (0.9848 and 0.9829 respectively). Interactions between the parameters had a strong synergistic effect on EOY and FY values as indicated by the 3D response surface curve and 2D contour. The experimental results matched the predicted results closely. This indicated the suitability of the models developed and the success of FCCCD under RSM in optimizing the S. Binderi extraction conditions.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(07)%202017%20supp...

Abstract

Supercritical carbon dioxide (SC-CO) extraction of fucoxanthin is more advantageous over conventional solvent extraction as it is less toxic less hazardous to the environment and preserves the bioactivity of fucoxanthin. A face-centered central composite design (FCCCD) based on response surface methodology (RSM) was employed for SC-CO extraction of oils and fucoxanthin from the brown seaweed Sargassum binderi with ethanol as a co- solvent. Three independent parameters namely extraction temperature (A: 40 50 60C) pressure (B:2900 3625 4350 psig and particle size (C: 90 500 and 1000 m) were investigated to optimize extraction oil yields (EOY) and fucoxanthin yields (FY). A regression model was developed tested for quality of fit (R‚‚) and expressed in the form of 3D response surface curve and 2D contour. The optimum extraction conditions were obtained at extraction temperature (A) 50C pressure (B) 3625 psig and particle size (C) 500 m. Under these conditions optimal EOY and FY were 10.04 mg/g and 3188.99 g/g respectively. The difference between the lowest and the highest response in EOY and FY were 5.44 “ 10.04 mg/g and 2109.10 - 3188.90 g/g respectively. The lowest yields were identified at 60C 2900 psig and 1000 m. The regression models generated showing interactions between the variables and EOY and FY response were significant as tested by ANOVA (p 0.0005 and p 0.0007 respectively) with high R‚‚ values (0.9848 and 0.9829 respectively). Interactions between the parameters had a strong synergistic effect on EOY and FY values as indicated by the 3D response surface curve and 2D contour. The experimental results matched the predicted results closely. This indicated the suitability of the models developed and the success of FCCCD under RSM in optimizing the S. Binderi extraction conditions.

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Item Type:	Article
AGROVOC Term:	Sargassum
AGROVOC Term:	Brown seaweeds
AGROVOC Term:	Essential oils
AGROVOC Term:	Carotenoids
AGROVOC Term:	Carbon dioxide
AGROVOC Term:	Supercritical CO2 extraction
AGROVOC Term:	Extraction
AGROVOC Term:	Temperature
AGROVOC Term:	Pressure
AGROVOC Term:	Particle size
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 05:35
URI:	http://webagris.upm.edu.my/id/eprint/23977

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