Additional antioxidants on the determination of quercetin from Moringa oleifera leaves and variation content from different sources

Chansri N., . and Panya T., . and Sripanidkulchai B., . and Daodee S., . Additional antioxidants on the determination of quercetin from Moringa oleifera leaves and variation content from different sources. pp. 51-55. ISSN 2231-7546

The purpose of this study was to investigate the effect additional synthetic antioxidants have on the determination of quercetin from Moringa oleifera leaves and to determine the quercetin content in different sources of M.oleifera. Four antioxidants (tert-butylhydroquinone butylated hydroxytoluene butylated hydroxyanisole and ascorbic acid) were added in various amounts (2 5 and 10 mg) to the hydrolysis reaction used to analyze quercetin from crude ethanol extracts of M.oleifera leaves. The amount of quercetin recovered from each source was determined by HPLC method which was appropriate for analysis of quercetin levels in extracts as shown by validation criteria. It was found that addition of either single or mixed antioxidants to the hydrolysis reaction could increase the quantity of quercetin recovered from M.oleifera leaves. The amount of quercetin from seven plant materials varied from a low of 3.01 to a high of 13.43 mg/g crude extract. It could be concluded that the analysis of quercetin in M.oleifera leaves should add some of the antioxidant in the process of analysis such as BHA 5 mg and TBHQ 5 mg. This could confirm the actual amount found in the leave of this plant and promote the accurate and reliable results to use in the other studies.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/25%20(01)%202018/(7).pd...

Abstract

The purpose of this study was to investigate the effect additional synthetic antioxidants have on the determination of quercetin from Moringa oleifera leaves and to determine the quercetin content in different sources of M.oleifera. Four antioxidants (tert-butylhydroquinone butylated hydroxytoluene butylated hydroxyanisole and ascorbic acid) were added in various amounts (2 5 and 10 mg) to the hydrolysis reaction used to analyze quercetin from crude ethanol extracts of M.oleifera leaves. The amount of quercetin recovered from each source was determined by HPLC method which was appropriate for analysis of quercetin levels in extracts as shown by validation criteria. It was found that addition of either single or mixed antioxidants to the hydrolysis reaction could increase the quantity of quercetin recovered from M.oleifera leaves. The amount of quercetin from seven plant materials varied from a low of 3.01 to a high of 13.43 mg/g crude extract. It could be concluded that the analysis of quercetin in M.oleifera leaves should add some of the antioxidant in the process of analysis such as BHA 5 mg and TBHQ 5 mg. This could confirm the actual amount found in the leave of this plant and promote the accurate and reliable results to use in the other studies.

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Item Type:	Article
AGROVOC Term:	Moringa oleifera
AGROVOC Term:	Leaves
AGROVOC Term:	Antioxidants
AGROVOC Term:	Butylated hydroxytoluene
AGROVOC Term:	Butylated hydroxyanisole
AGROVOC Term:	Butylhydroquinone
AGROVOC Term:	Ascorbic acid
AGROVOC Term:	Extraction in chemical analysis
AGROVOC Term:	Hydrolysis
AGROVOC Term:	Quercetin
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23990

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