Quality performance of protein allergen isolates for allergy diagnostic test (Case: Indonesian soybeans (Glycine max) and peanuts (Arachis hypogaea))

Astuti R. M., . and Palupi N. S., . and Zakaria F. R., . Quality performance of protein allergen isolates for allergy diagnostic test (Case: Indonesian soybeans (Glycine max) and peanuts (Arachis hypogaea)). pp. 217-226. ISSN 2231-7546

Allergen isolate is used in skin prick test to assess the type of offending food for an allergic person. Peanut and soybean are some of the main food allergens. Some researchers showed that different cultivars and environmental conditions could alter IgE binding proteins profiles when tested against plasma from allergic individuals. Consequently the use of local soybean and peanut as allergen isolates for allergy diagnostic test (such as skin prick test) needed an experiment to characterize these raw materials for showing and proving that they were feasible as a sensitizer. This study aimed to isolate local soybean and peanut proteins and to characterize their allergic reactivity for using as allergen isolate. The protein isolate reactivity was tested by using SDS-PAGE electrophoresis immunoblotting and ELISA involving food allergic human sera containing IgE. It was found that the allergic proteins in soybean and peanut were specific for each individual. This is one of the benefits of using crude protein as an allergen diagnostic tool since a different person may have an allergy to different allergen proteins. Thus allergen isolates should be in a crude protein form. The results from ELISA analysis showed that the two protein isolates had a good sensitivity in detecting specific IgE thus seemed to be promising to be produced as an allergen isolates.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/25%20(01)%202018/(28).p...

Abstract

Allergen isolate is used in skin prick test to assess the type of offending food for an allergic person. Peanut and soybean are some of the main food allergens. Some researchers showed that different cultivars and environmental conditions could alter IgE binding proteins profiles when tested against plasma from allergic individuals. Consequently the use of local soybean and peanut as allergen isolates for allergy diagnostic test (such as skin prick test) needed an experiment to characterize these raw materials for showing and proving that they were feasible as a sensitizer. This study aimed to isolate local soybean and peanut proteins and to characterize their allergic reactivity for using as allergen isolate. The protein isolate reactivity was tested by using SDS-PAGE electrophoresis immunoblotting and ELISA involving food allergic human sera containing IgE. It was found that the allergic proteins in soybean and peanut were specific for each individual. This is one of the benefits of using crude protein as an allergen diagnostic tool since a different person may have an allergy to different allergen proteins. Thus allergen isolates should be in a crude protein form. The results from ELISA analysis showed that the two protein isolates had a good sensitivity in detecting specific IgE thus seemed to be promising to be produced as an allergen isolates.

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Item Type:	Article
AGROVOC Term:	Soybeans
AGROVOC Term:	Glycine max
AGROVOC Term:	Peanuts
AGROVOC Term:	Arachis hypogaea
AGROVOC Term:	Allergens
AGROVOC Term:	Protein isolates
AGROVOC Term:	Electrophoresis
AGROVOC Term:	Immunoblotting
AGROVOC Term:	ELISA
AGROVOC Term:	Crude protein
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/24009

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