Comparison between morphophysiological and molecular methods for the identification of yeasts isolated from honey

Soares M. J. S., . and Muratori M. C. S., . and Nóbrega M. M. G. P., . and Poli J. S., . and Santos J. T. O., . and Pinheiro R. E. E., . and Rodrigues A. M. D., . and Costa J. A., . and Rosa C. A., . Comparison between morphophysiological and molecular methods for the identification of yeasts isolated from honey. pp. 418-422. ISSN 2231-7546

Honey has a characteristic microbiological profile depending on their physical and chemical composition results of the concentration sugar with a high degree of resistance to the proliferation of microorganisms. Thus the aim of the present study was to compare the morphophysiological and molecular methods for identification of yeast isolated from the honey obtained from the Apis mellifera L. bee. To this end 97 samples of floral honey acquired at two of Piau­s Brazil honey cooperatives were analyzed. Yeast identification was conducted by the morphophysiological method and by the molecular analysis of the sequences of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region. Six yeast strains were isolated two of them new isolated species; however comparisons between the morphophysiological and molecular methods showed divergence in the identification all the isolated species. Thus it is concluded that morphophysiological identification tests alone are unreliable as a single yeast identification means and that molecular methods showed higher effectiveness in the identification of yeast isolates.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/25%20(01)%202018/(55).p...

Abstract

Honey has a characteristic microbiological profile depending on their physical and chemical composition results of the concentration sugar with a high degree of resistance to the proliferation of microorganisms. Thus the aim of the present study was to compare the morphophysiological and molecular methods for identification of yeast isolated from the honey obtained from the Apis mellifera L. bee. To this end 97 samples of floral honey acquired at two of Piau­s Brazil honey cooperatives were analyzed. Yeast identification was conducted by the morphophysiological method and by the molecular analysis of the sequences of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region. Six yeast strains were isolated two of them new isolated species; however comparisons between the morphophysiological and molecular methods showed divergence in the identification all the isolated species. Thus it is concluded that morphophysiological identification tests alone are unreliable as a single yeast identification means and that molecular methods showed higher effectiveness in the identification of yeast isolates.

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Item Type:	Article
AGROVOC Term:	Honey bees
AGROVOC Term:	Apis mellifera ligustica
AGROVOC Term:	Honey
AGROVOC Term:	Beekeeping
AGROVOC Term:	Yeasts
AGROVOC Term:	Identification
AGROVOC Term:	Isolation techniques
AGROVOC Term:	genomics
AGROVOC Term:	morphology
AGROVOC Term:	Physiological adaptation
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/24033

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