Laboratory assessment of affordable culture media for the propagation of entomopathogenic fungi used in mycopesticide production


Citation

Anitha S., . and Das S. Sam Manohar, . Laboratory assessment of affordable culture media for the propagation of entomopathogenic fungi used in mycopesticide production. pp. 33-42. ISSN 2180-1983

Abstract

Attempts are made to identify novel materials for the mass production of entomopathogenic fungi Metarhizium anisopliae (Mets.) which are extremely cheap and can be used as alternative media components. Wheat bran rice bran tamarind extract and milled rice have been tested as cheap growth media. Among the five different media tested highest wet and dry biomass of 25.20.13g/100ml and 1.150.1 g/100ml was recorded for the milled rice medium whereas the least was observed for rice bran medium. Significant differences in conidiospore production were observed for the different cheap media. Tamarind solid media recorded the maximum spore count of 27.8 x104 spores/ml. The viability and spore count was higher in the solid media compared to the broth media. Cheap media and low-tech mass production methods have to be developed so as to supply the fungal pesticides at an affordable cost.


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Abstract

Attempts are made to identify novel materials for the mass production of entomopathogenic fungi Metarhizium anisopliae (Mets.) which are extremely cheap and can be used as alternative media components. Wheat bran rice bran tamarind extract and milled rice have been tested as cheap growth media. Among the five different media tested highest wet and dry biomass of 25.20.13g/100ml and 1.150.1 g/100ml was recorded for the milled rice medium whereas the least was observed for rice bran medium. Significant differences in conidiospore production were observed for the different cheap media. Tamarind solid media recorded the maximum spore count of 27.8 x104 spores/ml. The viability and spore count was higher in the solid media compared to the broth media. Cheap media and low-tech mass production methods have to be developed so as to supply the fungal pesticides at an affordable cost.

Additional Metadata

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Item Type: Article
AGROVOC Term: Metarhizium anisopliae
AGROVOC Term: Entomopathogenic fungi
AGROVOC Term: Biological control
AGROVOC Term: Biological control agents
AGROVOC Term: Culture media
AGROVOC Term: Growth media
AGROVOC Term: Natural enemies
AGROVOC Term: Laboratory experimentation
AGROVOC Term: Potatoes
AGROVOC Term: Tamarind
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:28
URI: http://webagris.upm.edu.my/id/eprint/24077

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