Citation
Salma S., . and Sarmiad W., . and Susilowati D. N., . and Lestari P., . and Richana N, . and Suryadi Y., . Evaluation of cellulase activity from Trichoderma spp. and Xylanolytic bacteria. pp. 1-16. ISSN 2180-1983
Abstract
Fungi and bacteria were considered as potential decomposers on various substrates. This study was aimed to evaluate the ability of Trichoderma spp. to degrade various cellulolytic substrates and xylanolytic bacteria to decompose lignocelluloses from rice straw. Six selected Trichoderma spp. isolates showed high activities of specific celullase in given substrates. Pan23.2 isolate was shown to be superior in all cellulose substrates without glucose with the highest activity to hydrolyze cellulose crystals. Some isolates preferentially hydrolyzed crystalline (Pan23.2 Bo17 Andi2.1) while others (Kun4 Gam4.1 Pan23.1) were more dominant on amorph hydrolysis. Not all cellulases from Trichoderma spp. with high activity on cellulose amorph showed activity on cellulose crystals. The addition of glucose into cellulose substrates increased the cellulase productivity of Andi2.1 and Gam4.1 isolates. Combination of 1 glucose and 1 cellobiose were the best substrate for CMCase activity while 1 glucose and 1 CMC were good for avicelase. However xylanolytic bacteria had no ability to degrade lignocelluloses on rice straw which was indicated by low temperature and high C/N ratio during composting process. In this study all Trichoderma spp. isolates demonstrated high potency as decomposers but the xylanolytic bacteria may be used for other applications other than as decomposers.
Download File
Full text available from:
Official URL: https://journal.unisza.edu.my/agrobiotechnology/in...
|
Abstract
Fungi and bacteria were considered as potential decomposers on various substrates. This study was aimed to evaluate the ability of Trichoderma spp. to degrade various cellulolytic substrates and xylanolytic bacteria to decompose lignocelluloses from rice straw. Six selected Trichoderma spp. isolates showed high activities of specific celullase in given substrates. Pan23.2 isolate was shown to be superior in all cellulose substrates without glucose with the highest activity to hydrolyze cellulose crystals. Some isolates preferentially hydrolyzed crystalline (Pan23.2 Bo17 Andi2.1) while others (Kun4 Gam4.1 Pan23.1) were more dominant on amorph hydrolysis. Not all cellulases from Trichoderma spp. with high activity on cellulose amorph showed activity on cellulose crystals. The addition of glucose into cellulose substrates increased the cellulase productivity of Andi2.1 and Gam4.1 isolates. Combination of 1 glucose and 1 cellobiose were the best substrate for CMCase activity while 1 glucose and 1 CMC were good for avicelase. However xylanolytic bacteria had no ability to degrade lignocelluloses on rice straw which was indicated by low temperature and high C/N ratio during composting process. In this study all Trichoderma spp. isolates demonstrated high potency as decomposers but the xylanolytic bacteria may be used for other applications other than as decomposers.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Trichoderma |
| AGROVOC Term: | Xylanolytic microorganisms |
| AGROVOC Term: | Cellulase |
| AGROVOC Term: | Cellulose |
| AGROVOC Term: | Lignocellulose |
| AGROVOC Term: | Hemicellulose |
| AGROVOC Term: | Rice straw |
| AGROVOC Term: | Bacteria |
| AGROVOC Term: | Enzymes |
| AGROVOC Term: | Enzyme activity |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:28 |
| URI: | http://webagris.upm.edu.my/id/eprint/24081 |
Actions (login required)
 |
View Item |
