Short communication: genomic constitution of oil palm interspecific hybrid crosses monitored by genomic in situ hybridisation (GISH)

Nordiana Hanim Mohd Nor, . and Xaviar Arulandoo, . and Muhammad Azwan Zulkifli, . and Maria Madon, . and Kandha Sritharan, . and Mohd Zaki N., . Short communication: genomic constitution of oil palm interspecific hybrid crosses monitored by genomic in situ hybridisation (GISH). pp. 338-344. ISSN 1511-2780

Interspecific hybridisation of Elaeis oleifera (O) and Elaeis guineensis (G) oil palm was done to create recombinant progenies containing various composition of parental genetic material. Genomic in situ hybridisation (GISH) on OxG hybrids had previously shown presence of 16 E. oleifera and 16 E. guineensis chromosomes. In this study GISH was used to examine the metaphase chromosomes of the OxG interspecific hybrids hybridised with E. guineensis (OxG)xG to produce backcross one (BC1) progenies. Optimisation of unlabelled E. guineensis genomic DNA quantity were required to produce optimum discrimination of the E. oleifera and E. guineensis chromosomes. The amount needed was 120 g indicating the high genome similarity. The chromosomes of BC1 individuals were 2n32 and consisted of variable composition of E. oleifera and E. guineensis chromosomes while the interphase nuclei showed regions of parental genomes in discrete non-intermixed domains indicating non-random organisation of the nucleus. The seedlings were created to assist in GISH analysis hence only leaflet plane correlation with the number of E. oleifera chromosomes transmitted into the progenies were done. No correlation can be observed between the numbers of E. oleifera chromosomes and the leaflet plane.

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Abstract

Interspecific hybridisation of Elaeis oleifera (O) and Elaeis guineensis (G) oil palm was done to create recombinant progenies containing various composition of parental genetic material. Genomic in situ hybridisation (GISH) on OxG hybrids had previously shown presence of 16 E. oleifera and 16 E. guineensis chromosomes. In this study GISH was used to examine the metaphase chromosomes of the OxG interspecific hybrids hybridised with E. guineensis (OxG)xG to produce backcross one (BC1) progenies. Optimisation of unlabelled E. guineensis genomic DNA quantity were required to produce optimum discrimination of the E. oleifera and E. guineensis chromosomes. The amount needed was 120 g indicating the high genome similarity. The chromosomes of BC1 individuals were 2n32 and consisted of variable composition of E. oleifera and E. guineensis chromosomes while the interphase nuclei showed regions of parental genomes in discrete non-intermixed domains indicating non-random organisation of the nucleus. The seedlings were created to assist in GISH analysis hence only leaflet plane correlation with the number of E. oleifera chromosomes transmitted into the progenies were done. No correlation can be observed between the numbers of E. oleifera chromosomes and the leaflet plane.

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Item Type:	Article
AGROVOC Term:	Oil palm
AGROVOC Term:	Interspecific hybridization
AGROVOC Term:	Backcrossing
AGROVOC Term:	Plant breeding
AGROVOC Term:	Hybrids
AGROVOC Term:	Hybridization
AGROVOC Term:	Chromosomes
AGROVOC Term:	Planting
AGROVOC Term:	Seedlings
AGROVOC Term:	DNA
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 06:05
URI:	http://webagris.upm.edu.my/id/eprint/24084

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