Citation
Peck Loo K., . and Mashitah M. D., . Extraction of acid and pepsin soluble collagen from selected Malaysian freshwater fish muscles: modified lowrys measurement method. pp. 17-32. ISSN 2180-1983
Abstract
Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) were extracted from the muscles of selected cultured freshwater fishes that are widely consumed as food in Malaysia. The species tested were Clarias species (hybrid of Clarias gariepinus C. macrocephalus) black tilapia (Oreochromis mossambicus) pangasius catfish (Pangasius sutchi) and sultan fish (Leptobarbus hoevenii). The concentrations of extracted collagen were determined by using Lowrys method with slight modification. Results showed that such modification was possible for measurement of collagen concentration directly from the extracting medium. The highest difference between the yields of ASC and PSC was found in Clarias spp. which was approximately 3.8-fold though no significant increase in the yields of both collagens was achieved after 12 hours of the extraction period. At the end of the extraction process the range of yields based on dry weight of muscles of ASC and PSC for all of the selected fish muscles were between 97.52 0.97 to 139.71 0.18 mg/g and 299.93 0.45 to 368.36 1.05 mg/g respectively. The presence of collagen in fish muscles as reported in this study showed that these Malaysian freshwater fishes have a potential to be utilized as alternative sources of mammalian collagens.
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Abstract
Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) were extracted from the muscles of selected cultured freshwater fishes that are widely consumed as food in Malaysia. The species tested were Clarias species (hybrid of Clarias gariepinus C. macrocephalus) black tilapia (Oreochromis mossambicus) pangasius catfish (Pangasius sutchi) and sultan fish (Leptobarbus hoevenii). The concentrations of extracted collagen were determined by using Lowrys method with slight modification. Results showed that such modification was possible for measurement of collagen concentration directly from the extracting medium. The highest difference between the yields of ASC and PSC was found in Clarias spp. which was approximately 3.8-fold though no significant increase in the yields of both collagens was achieved after 12 hours of the extraction period. At the end of the extraction process the range of yields based on dry weight of muscles of ASC and PSC for all of the selected fish muscles were between 97.52 0.97 to 139.71 0.18 mg/g and 299.93 0.45 to 368.36 1.05 mg/g respectively. The presence of collagen in fish muscles as reported in this study showed that these Malaysian freshwater fishes have a potential to be utilized as alternative sources of mammalian collagens.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Clarias gariepinus |
| AGROVOC Term: | Oreochromis mossambicus |
| AGROVOC Term: | Pangasius |
| AGROVOC Term: | Leptobarbus hoeveni |
| AGROVOC Term: | Freshwater fishes |
| AGROVOC Term: | Extraction |
| AGROVOC Term: | Pepsin |
| AGROVOC Term: | Isolation |
| AGROVOC Term: | Measurement |
| AGROVOC Term: | Absorbance |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 06:05 |
| URI: | http://webagris.upm.edu.my/id/eprint/24095 |
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