Internal Transcribed Spacer (ITS) as a molecular marker for identification of Dioscorea hispida dennst. (Dioscoreaceae)

Nor Zuhailah Mazlan, . and Nashriyah Mat, . and Salmah Tajuddin, . and Norhayati Ngah, . and Munirah Samsuddin, . and A. M. Ali, . and Siti Nor Indah Omar, . Internal Transcribed Spacer (ITS) as a molecular marker for identification of Dioscorea hispida dennst. (Dioscoreaceae). pp. 47-55. ISSN 2180-1983

Dioscorea hispida is a source of food and also has important medicinal values. However there are difficulties in identifying and recognizing D. hispida based on morphological characteristics. D. hispida collected from Besut District in the state of Terengganu Malaysia were subjected to PCR amplification with ITS1 and ITS2 (universal primers) but no amplified product was obtained. Therefore a new set of primers was designed based on a specific signature sequence for Dioscorea spp. in the ITS region named as Dios_003 (5-AAA CGG CTA CCA CAT CCA AG-3) and Dios_004 (5-AGA CAA ATC GCT CCA CCA AC-3). A specific band of 900 bp was detected for all accession numbers of D. hispida. Sequencing of the amplified ITS region was carried out and alignment of the sequences demonstrated that the ITS region amplified was identical in all the D. hispida accessions and was highly conserved among other Dioscorea spp. that were analyzed i.e. D. japonica D. alata D. elephantipes D. communis D. caucasia D. sylvatica and D. polystachya. Therefore the new set of internal transcribed spacer (ITS) marker is an effective marker to identify D. hispida.

Full text available from: Official URL: https://journal.unisza.edu.my/agrobiotechnology/in...

Abstract

Dioscorea hispida is a source of food and also has important medicinal values. However there are difficulties in identifying and recognizing D. hispida based on morphological characteristics. D. hispida collected from Besut District in the state of Terengganu Malaysia were subjected to PCR amplification with ITS1 and ITS2 (universal primers) but no amplified product was obtained. Therefore a new set of primers was designed based on a specific signature sequence for Dioscorea spp. in the ITS region named as Dios_003 (5-AAA CGG CTA CCA CAT CCA AG-3) and Dios_004 (5-AGA CAA ATC GCT CCA CCA AC-3). A specific band of 900 bp was detected for all accession numbers of D. hispida. Sequencing of the amplified ITS region was carried out and alignment of the sequences demonstrated that the ITS region amplified was identical in all the D. hispida accessions and was highly conserved among other Dioscorea spp. that were analyzed i.e. D. japonica D. alata D. elephantipes D. communis D. caucasia D. sylvatica and D. polystachya. Therefore the new set of internal transcribed spacer (ITS) marker is an effective marker to identify D. hispida.

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Item Type:	Article
AGROVOC Term:	Dioscorea hispida
AGROVOC Term:	Dioscoreaceae
AGROVOC Term:	Molecular markers
AGROVOC Term:	Genes
AGROVOC Term:	Genetic markers
AGROVOC Term:	Identification
AGROVOC Term:	DNA
AGROVOC Term:	Extraction
AGROVOC Term:	Purification
AGROVOC Term:	Leaf extracts
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 06:06
URI:	http://webagris.upm.edu.my/id/eprint/24105

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