Citation
Nor Zuhailah Mazlan, . and Nashriyah Mat, . and Salmah Tajuddin, . and Norhayati Ngah, . and Munirah Samsuddin, . and A. M. Ali, . and Siti Nor Indah Omar, . Internal Transcribed Spacer (ITS) as a molecular marker for identification of Dioscorea hispida dennst. (Dioscoreaceae). pp. 47-55. ISSN 2180-1983
Abstract
Dioscorea hispida is a source of food and also has important medicinal values. However there are difficulties in identifying and recognizing D. hispida based on morphological characteristics. D. hispida collected from Besut District in the state of Terengganu Malaysia were subjected to PCR amplification with ITS1 and ITS2 (universal primers) but no amplified product was obtained. Therefore a new set of primers was designed based on a specific signature sequence for Dioscorea spp. in the ITS region named as Dios_003 (5-AAA CGG CTA CCA CAT CCA AG-3) and Dios_004 (5-AGA CAA ATC GCT CCA CCA AC-3). A specific band of 900 bp was detected for all accession numbers of D. hispida. Sequencing of the amplified ITS region was carried out and alignment of the sequences demonstrated that the ITS region amplified was identical in all the D. hispida accessions and was highly conserved among other Dioscorea spp. that were analyzed i.e. D. japonica D. alata D. elephantipes D. communis D. caucasia D. sylvatica and D. polystachya. Therefore the new set of internal transcribed spacer (ITS) marker is an effective marker to identify D. hispida.
Download File
Full text available from:
Official URL: https://journal.unisza.edu.my/agrobiotechnology/in...
|
Abstract
Dioscorea hispida is a source of food and also has important medicinal values. However there are difficulties in identifying and recognizing D. hispida based on morphological characteristics. D. hispida collected from Besut District in the state of Terengganu Malaysia were subjected to PCR amplification with ITS1 and ITS2 (universal primers) but no amplified product was obtained. Therefore a new set of primers was designed based on a specific signature sequence for Dioscorea spp. in the ITS region named as Dios_003 (5-AAA CGG CTA CCA CAT CCA AG-3) and Dios_004 (5-AGA CAA ATC GCT CCA CCA AC-3). A specific band of 900 bp was detected for all accession numbers of D. hispida. Sequencing of the amplified ITS region was carried out and alignment of the sequences demonstrated that the ITS region amplified was identical in all the D. hispida accessions and was highly conserved among other Dioscorea spp. that were analyzed i.e. D. japonica D. alata D. elephantipes D. communis D. caucasia D. sylvatica and D. polystachya. Therefore the new set of internal transcribed spacer (ITS) marker is an effective marker to identify D. hispida.
Additional Metadata
Item Type: | Article |
---|---|
AGROVOC Term: | Dioscorea hispida |
AGROVOC Term: | Dioscoreaceae |
AGROVOC Term: | Molecular markers |
AGROVOC Term: | Genes |
AGROVOC Term: | Genetic markers |
AGROVOC Term: | Identification |
AGROVOC Term: | DNA |
AGROVOC Term: | Extraction |
AGROVOC Term: | Purification |
AGROVOC Term: | Leaf extracts |
Geographical Term: | Malaysia |
Depositing User: | Ms. Suzila Mohamad Kasim |
Last Modified: | 28 Apr 2025 06:06 |
URI: | http://webagris.upm.edu.my/id/eprint/24105 |
Actions (login required)
![]() |
View Item |