Effects of different stages of cryopreservation of red tilapia (Oreochromis niloticus) sperm and the variability between three individual fish in response to cryopreservation


Citation

Asmad K., . and Wan Khadijah W. E., . and Abdullah R. B., . Effects of different stages of cryopreservation of red tilapia (Oreochromis niloticus) sperm and the variability between three individual fish in response to cryopreservation. pp. 25-33. ISSN 2180-1983

Abstract

Cryopreservation is a technique that makes long term storage of fish semen possible. The objectives of the present study were to determine the effects of different stages of cryopreservation and individual variability of fish in the cryopreservation process. This study involved five stages of cryopreservation (fresh sperm sperm extender after equilibration time after vapor phase and after freezing) and three different fish. Sperm movement and velocity distribution for fresh and after frozen-thawed semen were evaluated using an automated semen analyzer. The motility of red tilapia fish sperm decreased gradually in the freezing process. The highest value of motility was obtained at fresh sperm (96.93 0.53) followed by sperm with extender (92.64 1.05) after equilibration time (84.86 2.26) and after vapor phase (67.82 3.28) whilst the lowest value of motility was obtained for sperm after freezing (61.39 3.62). For individual fish Fish 1 significantly showed the highest total motility (77.50 1.86).


Download File

Full text available from:

Abstract

Cryopreservation is a technique that makes long term storage of fish semen possible. The objectives of the present study were to determine the effects of different stages of cryopreservation and individual variability of fish in the cryopreservation process. This study involved five stages of cryopreservation (fresh sperm sperm extender after equilibration time after vapor phase and after freezing) and three different fish. Sperm movement and velocity distribution for fresh and after frozen-thawed semen were evaluated using an automated semen analyzer. The motility of red tilapia fish sperm decreased gradually in the freezing process. The highest value of motility was obtained at fresh sperm (96.93 0.53) followed by sperm with extender (92.64 1.05) after equilibration time (84.86 2.26) and after vapor phase (67.82 3.28) whilst the lowest value of motility was obtained for sperm after freezing (61.39 3.62). For individual fish Fish 1 significantly showed the highest total motility (77.50 1.86).

Additional Metadata

[error in script]
Item Type: Article
AGROVOC Term: Oreochromis niloticus
AGROVOC Term: Tilapia
AGROVOC Term: Aquatic animals
AGROVOC Term: Aquatic organisms
AGROVOC Term: Cryopreservation
AGROVOC Term: Sperm
AGROVOC Term: Semen
AGROVOC Term: Male animals
AGROVOC Term: Semen collection
AGROVOC Term: Semen diluents
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:28
URI: http://webagris.upm.edu.my/id/eprint/24106

Actions (login required)

View Item View Item