Citation
Fahmi A. B., . and Nor-Zuhailah M., . and Zaimah N. A. R., . and Norhaslinda H., . and Mahmod N. H., . and Nashriyah M., . and Fatihah N. H. N., . and A. M. Ali, . PCR-RFLP of Ficus deltoidea Jack (Moraceae). pp. 93-97. ISSN 2180-1983
Abstract
Previous taxonomic determination of Ficus deltoidea Jack was mainly based on narrative morphology. However the classification and relationship of some F. deltoidea varieties were disputable. In this study four universal primers were used to amplify chloroplast DNA and were digested with eight restriction enzymes to obtain intravarietal variation among Ficus deltoidea var. deltoidea Ficus deltoidea var. trengganuensis Ficus deltoidea var. intermedia Ficus deltoidea var. kunstleri Ficus deltoidea var. angustifolia and Ficus deltoidea var. bilobata. Pre-screening of six cpDNA primers revealed that the rbcL and rpoB amplified fragments contained restriction sites for HindIII and RsaI. However no polymorphism was observed by using this method.
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Abstract
Previous taxonomic determination of Ficus deltoidea Jack was mainly based on narrative morphology. However the classification and relationship of some F. deltoidea varieties were disputable. In this study four universal primers were used to amplify chloroplast DNA and were digested with eight restriction enzymes to obtain intravarietal variation among Ficus deltoidea var. deltoidea Ficus deltoidea var. trengganuensis Ficus deltoidea var. intermedia Ficus deltoidea var. kunstleri Ficus deltoidea var. angustifolia and Ficus deltoidea var. bilobata. Pre-screening of six cpDNA primers revealed that the rbcL and rpoB amplified fragments contained restriction sites for HindIII and RsaI. However no polymorphism was observed by using this method.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Ficus |
| AGROVOC Term: | Moraceae |
| AGROVOC Term: | morphology |
| AGROVOC Term: | Leaves |
| AGROVOC Term: | DNA |
| AGROVOC Term: | Isolation |
| AGROVOC Term: | Purification |
| AGROVOC Term: | Centrifugation |
| AGROVOC Term: | Genes |
| AGROVOC Term: | Ethanol |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 06:06 |
| URI: | http://webagris.upm.edu.my/id/eprint/24111 |
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