Profiling of anti-fungal activity of Trichoderma virens 159c involved in biocontrol assay of Ganoderma boninense

Mohd Termizi Yusof, . and Shamala Sundram, . and Intan Safinar Ismail, . and Lee A. P. L., . and Tay B. Y. P., . Profiling of anti-fungal activity of Trichoderma virens 159c involved in biocontrol assay of Ganoderma boninense. pp. 83-93. ISSN 1511-2780

Trichoderma has long been recognised as a potential biological control agent (BCA) against pathogenic fungi due to antagonistic characteristics and it has successfully controlled Ganoderma boninense at the nursery stage in previous study. This study attempts to identify the mechanisms involved in the suppression of G. boninense and the anti-fungal compounds released by endophytic Trichoderma virens 159c. Therefore culture filtrates of endophytic T. virens 159c was extracted using hexane ethyl acetate (EtOAc) and butanol (BuOH) and anti-fungal activity was tested. The EtOAc extract showed highest anti-fungal activity with percentage inhibition of radial growth (PIRG) of 78.39 5.40. The scanning electron microscope (SEM) showed severe deformation of G. boninense PER 71 mycelia observed at the inhibition region caused by EtOAc extract. Further fractionated with column chromatography and antifungal assay revealed that fractions 2 and 4 had highest anti-fungal activity. Analysis carried out using gas chromatography-mass spectrometry detector of the active fraction allowed the identification of acetamide alcohol lactones and free fatty acids. Phenylethyl alcohol (PEA) was discovered as a unique compound because the presence was only in the highly inhibitory fraction of T. virens. In addition dl-mevalonic acid lactone in fraction 4 of T. virens 159c was first reported in T. virens.

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Abstract

Trichoderma has long been recognised as a potential biological control agent (BCA) against pathogenic fungi due to antagonistic characteristics and it has successfully controlled Ganoderma boninense at the nursery stage in previous study. This study attempts to identify the mechanisms involved in the suppression of G. boninense and the anti-fungal compounds released by endophytic Trichoderma virens 159c. Therefore culture filtrates of endophytic T. virens 159c was extracted using hexane ethyl acetate (EtOAc) and butanol (BuOH) and anti-fungal activity was tested. The EtOAc extract showed highest anti-fungal activity with percentage inhibition of radial growth (PIRG) of 78.39 5.40. The scanning electron microscope (SEM) showed severe deformation of G. boninense PER 71 mycelia observed at the inhibition region caused by EtOAc extract. Further fractionated with column chromatography and antifungal assay revealed that fractions 2 and 4 had highest anti-fungal activity. Analysis carried out using gas chromatography-mass spectrometry detector of the active fraction allowed the identification of acetamide alcohol lactones and free fatty acids. Phenylethyl alcohol (PEA) was discovered as a unique compound because the presence was only in the highly inhibitory fraction of T. virens. In addition dl-mevalonic acid lactone in fraction 4 of T. virens 159c was first reported in T. virens.

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Item Type:	Article
AGROVOC Term:	Trichoderma
AGROVOC Term:	Ganoderma
AGROVOC Term:	Endophytes
AGROVOC Term:	Antifungal properties
AGROVOC Term:	Biological control agents
AGROVOC Term:	Fungal antagonists
AGROVOC Term:	Extracts
AGROVOC Term:	Isolation
AGROVOC Term:	Purification
AGROVOC Term:	Butanol
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 06:06
URI:	http://webagris.upm.edu.my/id/eprint/24114

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