Long-term storage of oil palm germplasm zygotic embryo using cryopreservation

Fadila Ahmad Malike, . and Marhalil Marjuni, . and Maizura Ithnin, . and Mohd Din Amiruddin, . and Norziha Abdullah, . and Rajanaidu N., . and Zulkifli Yaakub, . and Ahmad Kushairi, . Long-term storage of oil palm germplasm zygotic embryo using cryopreservation. pp. 541-547. ISSN 1511-2780

The MPOB germplasm collections from various parts of the world were planted mainly at MPOB Research Station Kluang Johor Malaysia. The collections were maintained in the form of field gene bank. Conserving the genetic materials in ex-situ living collections requires high cost of maintenance large land area and the palms are exposed to diseases and extreme weather conditions. Experiments were conducted to study the possibility of preserving oil palm tissues for long-term storage at ultra-low temperature (-196C) in liquid nitrogen. This technique known as cryopreservation requires lower cost of maintenance lesser space and protects the genetic materials from pests and diseases. Oil palm seeds are intermediate between orthodox and recalcitrant. Oil palm seeds that have high levels of moisture lose viability when stored in liquid nitrogen. Experiments were conducted to study the possibility of cryopreserving smaller tissues such as oil palm zygotic embryos. Simple desiccation methods were applied to reduce the moisture levels of the embryos namely room temperature laminar flow and silica gel. These methods were shown to be useful for zygotic embryos. Silica gel however is advantageous as it removes the moisture in relatively shorter period than the other two methods. The same approach has potential to be applied to cryopreserve oil palm pollen.

Full text available from: Official URL: http://jopr.mpob.gov.my/long-term-storage-of-oil-p...

Abstract

The MPOB germplasm collections from various parts of the world were planted mainly at MPOB Research Station Kluang Johor Malaysia. The collections were maintained in the form of field gene bank. Conserving the genetic materials in ex-situ living collections requires high cost of maintenance large land area and the palms are exposed to diseases and extreme weather conditions. Experiments were conducted to study the possibility of preserving oil palm tissues for long-term storage at ultra-low temperature (-196C) in liquid nitrogen. This technique known as cryopreservation requires lower cost of maintenance lesser space and protects the genetic materials from pests and diseases. Oil palm seeds are intermediate between orthodox and recalcitrant. Oil palm seeds that have high levels of moisture lose viability when stored in liquid nitrogen. Experiments were conducted to study the possibility of cryopreserving smaller tissues such as oil palm zygotic embryos. Simple desiccation methods were applied to reduce the moisture levels of the embryos namely room temperature laminar flow and silica gel. These methods were shown to be useful for zygotic embryos. Silica gel however is advantageous as it removes the moisture in relatively shorter period than the other two methods. The same approach has potential to be applied to cryopreserve oil palm pollen.

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Item Type:	Article
AGROVOC Term:	Cryopreservation
AGROVOC Term:	Oil palms
AGROVOC Term:	Plant embryos
AGROVOC Term:	Germplasm
AGROVOC Term:	Seed
AGROVOC Term:	Dehydration
AGROVOC Term:	Germplasm collections
AGROVOC Term:	Liquid nitrogen
AGROVOC Term:	Germination rate
AGROVOC Term:	Experiments
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 06:51
URI:	http://webagris.upm.edu.my/id/eprint/24129

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