Citation
Siti Suhaila A. R., . and Saleh N. M., . Inhibitory effect of Kanamycin on in vitro culture of Lycopersicon esculentum Mill cv. Mt11. pp. 79-86. ISSN 2180-1983
Abstract
Excised cotyledons of tomato (Lycopersicon esculentum Mill cv. MT11) were cultured on selective medium containing kanamycin at various concentrations (50 100 200 300 mg/L). Significant toxic effects were observed when the cotyledon explants were grown on MS medium supplemented with 5 mg/L kinetin and 100 mg/L kanamycin. The regeneration of callus was decreased as the concentration of kanamycin increased from 200 to 300 mg/L. Explants grown on MS medium supplemented with 5 mg/L kinetin and 50 mg/L kanamycin showed the least toxic effects (mean survival rate 48.0 0.19) compared to the rest of the concentrations tested. Even though 100 mg/L of kanamycin allows the non-transformed explants to grow on the medium the shoot primordia would not develop further. The result suggests that 100 mg/L of kanamycin can be used effectively to differentiate between non-transformed and transformed MT11 tomato explants with a death rate of more than 82 of non-transformed explants after 4 weeks of incubation on selection medium. Therefore 100 mg/L kanamycin is suitable for minimal inhibition concentration for MT11 and true transformants can be selected at this concentration for the transformation system.
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Abstract
Excised cotyledons of tomato (Lycopersicon esculentum Mill cv. MT11) were cultured on selective medium containing kanamycin at various concentrations (50 100 200 300 mg/L). Significant toxic effects were observed when the cotyledon explants were grown on MS medium supplemented with 5 mg/L kinetin and 100 mg/L kanamycin. The regeneration of callus was decreased as the concentration of kanamycin increased from 200 to 300 mg/L. Explants grown on MS medium supplemented with 5 mg/L kinetin and 50 mg/L kanamycin showed the least toxic effects (mean survival rate 48.0 0.19) compared to the rest of the concentrations tested. Even though 100 mg/L of kanamycin allows the non-transformed explants to grow on the medium the shoot primordia would not develop further. The result suggests that 100 mg/L of kanamycin can be used effectively to differentiate between non-transformed and transformed MT11 tomato explants with a death rate of more than 82 of non-transformed explants after 4 weeks of incubation on selection medium. Therefore 100 mg/L kanamycin is suitable for minimal inhibition concentration for MT11 and true transformants can be selected at this concentration for the transformation system.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Lycopersicon esculentum |
| AGROVOC Term: | Solanaceae |
| AGROVOC Term: | Kanamycin |
| AGROVOC Term: | Antibiotics |
| AGROVOC Term: | In vitro culture |
| AGROVOC Term: | Culture techniques |
| AGROVOC Term: | Explants |
| AGROVOC Term: | Seeds |
| AGROVOC Term: | Necrosis |
| AGROVOC Term: | Inhibition |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 06:52 |
| URI: | http://webagris.upm.edu.my/id/eprint/24134 |
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