Citation
Rodziah Kari, . and Ahmad Lutfi Lukman, . and Rokiah Zainuddin, . and Hafsah Ja’afar, . Basal media for in vitro germination of red-purple dragon fruit Hylocereus polyrhizus. pp. 87-93. ISSN 2180-1983
Abstract
Five types of basal media DM 94 (a modified MS) Gamborg B5 (1968) Murashige and Skoog (1962 MS) Lloyd and McCown (1980 LM) and Chinese A (a modified MS) were screened for germination of dragon fruit seeds obtained from the local supermarket. Based on preliminary experiments to determine the potential hormone combinations giving high germination percentage three combinations were selected. The treatments consisted of 0.8 ppm IBA 0.5 ppm IBA 1.0 ppm BAP 0.5 ppm IBA 1.0 ppm kinetin and control. Germination of seeds after three weeks in the media varied between 62.8 and 89.3. The highest and the lowest percentages came from similar treatment consisting of 0.5 ppm IBA 1.0 ppm kinetin but different basal media Chinese A and DM 94 respectively. The best medium for germination and subsequent growth was a modified MS called Chinese A which gave 100 transplanting success. This screening experiment showed that this medium can be used for future seed germination of red-purple dragon fruit Hylocereus polyrhizus.
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Abstract
Five types of basal media DM 94 (a modified MS) Gamborg B5 (1968) Murashige and Skoog (1962 MS) Lloyd and McCown (1980 LM) and Chinese A (a modified MS) were screened for germination of dragon fruit seeds obtained from the local supermarket. Based on preliminary experiments to determine the potential hormone combinations giving high germination percentage three combinations were selected. The treatments consisted of 0.8 ppm IBA 0.5 ppm IBA 1.0 ppm BAP 0.5 ppm IBA 1.0 ppm kinetin and control. Germination of seeds after three weeks in the media varied between 62.8 and 89.3. The highest and the lowest percentages came from similar treatment consisting of 0.5 ppm IBA 1.0 ppm kinetin but different basal media Chinese A and DM 94 respectively. The best medium for germination and subsequent growth was a modified MS called Chinese A which gave 100 transplanting success. This screening experiment showed that this medium can be used for future seed germination of red-purple dragon fruit Hylocereus polyrhizus.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Hylocereus |
| AGROVOC Term: | Culture media |
| AGROVOC Term: | Explants |
| AGROVOC Term: | In vitro |
| AGROVOC Term: | Growth |
| AGROVOC Term: | Micropropagation |
| AGROVOC Term: | Organic compounds |
| AGROVOC Term: | Sterilization |
| AGROVOC Term: | Experiments |
| AGROVOC Term: | IBA |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:28 |
| URI: | http://webagris.upm.edu.my/id/eprint/24135 |
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